Recombinant牛Growth Hormone protein (ab123464)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP01246
    • 种属Cow
    • 序列The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Phe-Pro-Ala.
    • 分子量22 kDa
    • 氨基酸27 to 217

技术指标

Our Abpromise guarantee covers the use of ab123464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性The activity as determined by the PDFP13B9 cells stably transfected with rabbit GH receptors. Bovine GH is also capable of forming a 1:2 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD).
  • 应用

    Functional Studies

    SDS-PAGE

    HPLC

  • 纯度> 98 % SDS-PAGE.
    ab123464 was purified by proprietary chromatographic techniques and filter sterilized. Greater than 98.0% as determined by SEC-HPLC and SDS-PAGE.
    Protein quantitation was carried out by two independent methods
    1. UV spectroscopy at 280 nm using the absorbency value of 0.68 as the extinction coefficient for a 0.1% (1mg/ml) solution.
    2. Analysis by RP-HPLC, using a calibrated solution of GH as a Reference Standard.
  • 形式Lyophilised
  • 补充说明Please prevent freeze-thaw cycles.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    pH: 8.00
    Constituent: 0.00004% Sodium bicarbonate

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶It is recommended to reconstitute ab123464 in sterile ultra pure water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

常规信息

  • 别名
    • gH
    • GH-N
    • GH1
    • GHN
    • Growth hormone
    • Growth hormone 1
    • Growth hormone, normal
    • Growth hormone, pituitary
    • HG1
    • hGH-N
    • IGHD1B
    • Pituitary growth hormone
    • RNGHGP
    • SOMA_HUMAN
    • Somatotropin
    see all
  • 功能Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
  • 疾病相关Defects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A) [MIM:262400]; also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH.
    Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
    Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
    Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy.
  • 序列相似性Belongs to the somatotropin/prolactin family.
  • 细胞定位Secreted.
  • Information by UniProt

Recombinant cow Growth Hormone protein (ab123464)参考文献

ab123464 has not yet been referenced specifically in any publications.

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