Recombinant牛Enterokinase protein (ab60893)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Cow

技术指标

Our Abpromise guarantee covers the use of ab60893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Functional Studies

  • 纯度> 95 % SDS-PAGE.

  • 形式Liquid
  • 补充说明This product is supplied as 1 vial containing ~0.2units of protein.


    This protein can be used for fusion protein cleavage. The rare 5 amino acid recognition sequence of the enterokinase cleavage site is incorporated into a tag with the sequence: N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C Cleavage by enterokinase removes the tag from the fusion protein.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50% Glycerol, 200mM Sodium chloride, 20mM Tris HCl, 2mM Calcium chloride

常规信息

  • 别名
    • PRSS 7
    • PRSS7
    • Enterokinase
    • Enterokinase catalytic subunit
    • Enteropeptidase
    • Enteropeptidase catalytic light chain
    • Enteropeptidase non catalytic heavy chain
    • ENTK
    • ENTK_HUMAN
    • PROENTEROKINASE
    • protease, serine, 7 (enterokinase)
    • PRSS7
    • Serine protease 7
    • TMPRSS15
    • Transmembrane protease serine 15
    see all
  • 功能Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.
  • 组织特异性Intestinal brush border.
  • 疾病相关Defects in TMPRSS15 are a cause of enterokinase deficiency (ENTKD) [MIM:226200]; a life-threatening intestinal malabsorption disorder characterized by diarrhea and failure to thrive.
  • 序列相似性Belongs to the peptidase S1 family.
    Contains 2 CUB domains.
    Contains 2 LDL-receptor class A domains.
    Contains 1 MAM domain.
    Contains 1 peptidase S1 domain.
    Contains 1 SEA domain.
    Contains 1 SRCR domain.
  • 翻译后修饰The chains are derived from a single precursor that is cleaved by a trypsin-like protease.
  • 细胞定位Membrane.
  • Information by UniProt

Recombinant Cow Enterokinase protein (ab60893)参考文献

ab60893 has not yet been referenced specifically in any publications.

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The enterokinase ab60893 is the recombinant protein from bovine intestine (based on the sequence SwissProt ID P98072, http://www.uniprot.org/uniprot/P98072).

ab68332 on the other hand is the recombinan...

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Thank you for your enquiry regarding ab60893. I can confirm that this product can be used for the cleavage of His-tags from recombinant proteins. I hope this helps and if I can assist further, please do not hesitate to contact me.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"