The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The biological activity of recombinant chicken IL10 was determined by measuring IFN-gamma expression by RTPCR in Concanavalin A-stimulated chicken spleen lymphocytes. Results demonstrate decreased IFN-gamma gene expression, with the addition of recombinant chicken IL10, in a dose dependant manner, with doses starting at 2 ng/ml.
% SDS-PAGE. ab87526 is purified by Ion-exchange chromatography.
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Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein.
Reconstituted protein is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze/thaw cycles.
Cytokine synthesis inhibitory factor
T-cell growth inhibitory factor
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.