Recombinant Arginine deiminase protein (ab119147)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP23793
    • 序列MSVFDSKFKGIHVYSEIGELESVLVHEPGREIDYITPARLDELLFSAILE SHDARKEHKQFVAELKANDINVVELIDLVAETYDLASQEAKDKLIEEFLE DSEPVLSEEHKVVVRNFLKAKKTSRELVEIMMAGITKTDLGIEADHELIV DPMPNLYFTRDPFASVGNGVTIHYMRYKVRQRETLFSRFVFSNHPKLINT PWYYDPSLKLSIEGGDVFIYNNDTLVVGVSERTDLQTVTLLAKNIVANKE CEFKRIVAINVPKWTNLMHLDTWLTMLDKDKFLYSPIANDVFKFWDYDLV NGGAEPQPVENGLPLEGLLQSIINKKPVLIPIAGEGASQMEIERETHFDG TNYLAIRPGVVIGYSRNEKTNAALEAAGIKVLPFHGNQLSLGMGNARCMS MPLSRKDVKW
    • 分子量47 kDa
    • 氨基酸1 to 410

技术指标

Our Abpromise guarantee covers the use of ab119147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Measured by its ability to induce apoptosis in Jurkat cells using a concentration of 100-150 ng/ml.
  • 应用

    SDS-PAGE

    HPLC

  • 内毒素水平< 0.100 Eu/µg
  • 纯度> 95 % SDS-PAGE.
    Purity greater than 97% by SDS-PAGE gel and HPLC analyses.
  • 形式Lyophilised
  • 补充说明


    Arginine Deiminase (ADI) is a microbial enzyme from Mycoplasma produced in E.coli. It has high affinity to L-arginine and hydrolyzes L-arginine to citrulline and ammonia. Low concentrations of ADI have been shown to inhibit proliferation in certain cultured cells by arresting the cell cycle in G (1) and/or S phase. Higher concentrations of ADI lead to subsequent apoptosis.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.03% Sodium phosphate, 0.003% DTT

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶Reconstitute in water to a concentration of 0.1-1.0 mg/ml.

常规信息

  • 别名
    • AD
    • ADI
    • Arginine deiminase
    • Arginine dihydrolase
    • EC=3.5.3.6
    see all
  • 相关性Amino-acid degradation; L-arginine degradation via ADI pathway; carbamoyl phosphate from L-arginine: step 1/2.
  • 细胞定位Cytoplasmic

Recombinant Arginine deiminase protein (ab119147)参考文献

ab119147 has not yet been referenced specifically in any publications.

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Reviewing the details for ab119147 Active Arginine deiminase full length protein, the datasheet gives a range between 100-150 ng/ml for induction of apoptosis in Jurkat cells. If you are determining the activity usi...

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