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Our Abpromise guarantee covers the use of ab84191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Endotoxin Level determined by LAL method
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 10% Glycerol, 20mM Tris HCl, pH 8.0
This product is an active protein and may elicit a biological response in vivo, handle with caution.
15% SDS-PAGE showing ab84191 at approximately 26.8kDa (3µg).
1. Prepare a 150 μl recombinant GFP with various concentrations (0.00074 nM - 6.21 μM) in assay buffer and equilibrate to 25°C. (Assay buffer: 10 mM Tris-HCl (pH 8.0), 10 mM EDTA, 0.02% sodium azide.)
2. Read at excitation wavelengths 485 nm and emission 535 nm.
- 96 Well Polystyrene Microplate, black
- Fluorescent plate reader
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"