Anti-RECK抗体(ab88249)
Key features and details
- Mouse polyclonal to RECK
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-RECK抗体
参阅全部 RECK 一抗 -
描述
小鼠多克隆抗体to RECK -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human RECK. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
-
阳性对照
- RECK transfected 293T cell line and RECK expression in 293 cell lysate
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab88249于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 106 kDa.
|
说明 |
---|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 106 kDa. |
靶标
-
功能
Negatively regulates matrix metalloproteinase-9 (MMP-9) by suppressing MMP-9 secretion and by direct inhibition of its enzymatic activity. RECK down-regulation by oncogenic signals may facilitate tumor invasion and metastasis. Appears to also regulate MMP-2 and MT1-MMP, which are involved in cancer progression. -
组织特异性
Expressed in various tissues and untransformed cells. It is undetectable in tumor-derived cell lines and oncogenically transformed cells. -
序列相似性
Contains 3 Kazal-like domains. -
翻译后修饰
N-glycosylated. -
细胞定位
Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 313488 Human
- Entrez Gene: 8434 Human
- Omim: 605227 Human
- SwissProt: O95980 Human
- SwissProt: Q5W0K6 Human
- Unigene: 728961 Human
-
别名
- hRECK antibody
- Membrane anchored glycoprotein (metastasis and invasion) antibody
- RECK antibody
see all
图片
-
Anti-RECK antibody (ab88249) at 1 µg/ml + 293 cell lysate at 50 µg
Predicted band size: 106 kDa -
All lanes : Anti-RECK antibody (ab88249) at 1 µg/ml
Lane 1 : RECK transfected 293T cell line
Lane 2 : Non-transfected 293T cell line
Lysates/proteins at 25 µg per lane.
Predicted band size: 106 kDa
Observed band size: 106 kDa
数据表及文件
-
Datasheet download
文献 (5)
ab88249 被引用在 5 文献中.
- Abdelkader NF et al. Empagliflozin mitigates type 2 diabetes-associated peripheral neuropathy: a glucose-independent effect through AMPK signaling. Arch Pharm Res 45:475-493 (2022). PubMed: 35767208
- Wei ZL et al. MicroRNA-221 promotes papillary thyroid carcinoma cell migration and invasion via targeting RECK and regulating epithelial-mesenchymal transition. Onco Targets Ther 12:2323-2333 (2019). PubMed: 30992669
- Iseki Y et al. MicroRNA-96 Promotes Tumor Invasion in Colorectal Cancer via RECK. Anticancer Res 38:2031-2035 (2018). PubMed: 29599320
- Liu W et al. miR-221 and miR-222 Simultaneously Target RECK and Regulate Growth and Invasion of Gastric Cancer Cells. Med Sci Monit 21:2718-25 (2015). WB ; Human . PubMed: 26364844
- Zhou XQ et al. Epigallocatechin-3-gallate inhibits the invasion of salivary adenoid cystic carcinoma cells by reversing the hypermethylation status of the RECK gene. Mol Med Rep 12:6031-6 (2015). PubMed: 26299812