概述

  • 产品名称
    Anti-RecA抗体
  • 描述
    兔多克隆抗体to RecA
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Escherichia coli
  • 免疫原

    Recombinant full length RecA (Escherichia coli) highly purified by chromatography.

  • 阳性对照
    • Extract of E.coli AB1157.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab63797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/3000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa).
IP 1/600.

靶标

  • 相关性
    RecA in E.coli can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation and leading to its autocatalytic cleavage.
  • 细胞定位
    Cytoplasmic
  • 数据库链接
    • 别名
      • DNA strand exchange and recombination protein with protease and nuclease activity antibody
      • lexB antibody
      • Protein recA antibody
      • recH antibody
      • Recombinase A antibody
      see all

    图片

    • Immunoprecipitation - Anti-RecA antibody (ab63797)
      Immunoprecipitation - Anti-RecA antibody (ab63797)

      Immunoprecipitation of RecA protein from crudeextract of E. coli cells.  2 μg of ab63797 was adsorbed to proteinA magnetic beads to precipitate RecA protein from supernatant of sonic disrupted E. coli cells. The precipitate was analyzed by western blot.

    • Western blot - Anti-RecA antibody (ab63797)
      Western blot - Anti-RecA antibody (ab63797)
      All lanes : Anti-RecA antibody (ab63797) at 1/3000 dilution

      Lane 1 : Molecular weight markers
      Lane 2 : Purified RecA
      Lane 3 : Extract of E.coli AB1157

      Predicted band size: 38 kDa
      Observed band size: 42 kDa (why is the actual band size different from the predicted?)

    文献

    This product has been referenced in:
    • Irazoki O  et al. The Transient Multidrug Resistance Phenotype of Salmonella enterica Swarming Cells Is Abolished by Sub-inhibitory Concentrations of Antimicrobial Compounds. Front Microbiol 8:1360 (2017). Read more (PubMed: 28769915) »
    • Pinaud L  et al. Identification of novel substrates of Shigella T3SA through analysis of its virulence plasmid-encoded secretome. PLoS One 12:e0186920 (2017). Read more (PubMed: 29073283) »

    See all 14 Publications for this product

    发表研究结果有使用 ab63797？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

    提交评价 提交问题

    5 reviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-RecA antibody

     Average
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Neisseria meningitidis Cell lysate - whole cell (bacteria)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    1e+006 cells
    Specification
    bacteria
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    提交于 Mar 29 2018

    Western blot abreview for Anti-RecA antibody

     Excellent
    Abreviews
    Application
    Western blot
    Loading amount
    1000 µg
    Gel Running Conditions
    Reduced Denaturing (13)
    Sample
    Barley (Hordeum vulgare) Tissue lysate - other (embryos and leaves soluble fraction of thylakoids)
    Specification
    embryos and leaves soluble fraction of thylakoids
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    abreview image
    Username

    Dr. Franco Lencina

    Verified customer

    提交于 Jul 29 2014

    Western blot abreview for Anti-RecA antibody

     Excellent
    Abreviews
    Application
    Western blot
    Sample
    Pseudoalteromonas luteoviolacea Cell lysate - whole cell (whole cell)
    Loading amount
    10 µg
    Specification
    whole cell
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Read More
    Username

    Abcam user community

    Verified customer

    提交于 Apr 15 2013

    I would like to know whether there is enough homology between my protein and the target epitope of this antibody (ab63797, Anti-RecA from E. coli) to be sufficient for me to use the antibody in a western blot assay. The sequence of my protein is below....

    Read More

    BLAST alignment of your sequence with the sequence of the immunogen for this antibody, full-length E. coli RecA, (from the UniProt entry at this link: http://www.uniprot.org/uniprot/P0A7G6) returns a result of 77% identity. Since the antibody is polycl...

    Read More

    Can this product be used to detect the recA in V. cholerae?

    Thank you for contacting us. It is highly likely that RecA protein of V. cholerae can be detected by immunoblotting with ab63797 because of the high sequence similarity of RecA protein between V. cholerae and E. Coli while the immunogen is whol...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册