Customer reviews and FAQs

概述

  • 产品名称Anti-RecA抗体
  • 描述
    兔多克隆抗体to RecA
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Escherichia coli
  • 免疫原

    Recombinant full length RecA (Escherichia coli) highly purified by chromatography.

  • 阳性对照
    • Extract of E.coli AB1157.

性能

应用

Our Abpromise guarantee covers the use of ab63797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/3000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa).
IP 1/600.

靶标

  • 相关性RecA in E.coli can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation and leading to its autocatalytic cleavage.
  • 细胞定位Cytoplasmic
  • 数据库链接
    • 别名
      • DNA strand exchange and recombination protein with protease and nuclease activity antibody
      • lexB antibody
      • Protein recA antibody
      • recH antibody
      • Recombinase A antibody
      see all

    Anti-RecA antibody 图像

    • Immunoprecipitation - Anti-RecA antibody (ab63797)
      Immunoprecipitation - Anti-RecA antibody (ab63797)

      Immunoprecipitation of RecA protein from crudeextract of E. coli cells.  2 μg of ab63797 was adsorbed to proteinA magnetic beads to precipitate RecA protein from supernatant of sonic disrupted E. coli cells. The precipitate was analyzed by western blot.

    • Western blot - RecA antibody (ab63797)
      Western blot - RecA antibody (ab63797)
      All lanes : Anti-RecA antibody (ab63797) at 1/3000 dilution

      Lane 1 : Molecular weight markers
      Lane 2 : Purified RecA
      Lane 3 : Extract of E.coli AB1157


      Predicted band size : 38 kDa
      Observed band size : 42 kDa (why is the actual band size different from the predicted?)

    Anti-RecA antibody (ab63797)参考文献

    This product has been referenced in:
    • Naiman K  et al. A defect in homologous recombination leads to increased translesion synthesis in E. coli. Nucleic Acids Res 44:7691-9 (2016). Read more (PubMed: 27257075) »
    • Naiman K  et al. Chronology in lesion tolerance gives priority to genetic variability. Proc Natl Acad Sci U S A 111:5526-31 (2014). Read more (PubMed: 24706928) »

    See all 7 Publications for this product

    发表研究结果有使用 ab63797？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    提交 ab63797 的 abreview 提交 ab63797 的问题

    4 Abreviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-RecA antibody

     Excellent
    Abreviews
    Application Western blot
    Loading amount 1000 µg
    Gel Running Conditions Reduced Denaturing (13)
    Sample Barley (Hordeum vulgare) Tissue lysate - other (embryos and leaves soluble fraction of thylakoids)
    Specification embryos and leaves soluble fraction of thylakoids
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
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    Username

    Dr. Franco Lencina

    Verified customer

    提交于 Jul 29 2014

    Western blot abreview for Anti-RecA antibody

     Excellent
    Abreviews
    Application Western blot
    Sample Pseudoalteromonas luteoviolacea Cell lysate - whole cell (whole cell)
    Loading amount 10 µg
    Specification whole cell
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Read More
    Username

    Abcam user community

    Verified customer

    提交于 Apr 15 2013

    I would like to know whether there is enough homology between my protein and the target epitope of this antibody (ab63797, Anti-RecA from E. coli) to be sufficient for me to use the antibody in a western blot assay. The sequence of my protein is below....

    Read More

    BLAST alignment of your sequence with the sequence of the immunogen for this antibody, full-length E. coli RecA, (from the UniProt entry at this link: http://www.uniprot.org/uniprot/P0A7G6) returns a result of 77% identity. Since the antibody is polycl...

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    Can this product be used to detect the recA in V. cholerae?

    Thank you for contacting us. It is highly likely that RecA protein of V. cholerae can be detected by immunoblotting with ab63797 because of the high sequence similarity of RecA protein between V. cholerae and E. Coli while the immunogen is whol...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"