Anti-REC8 (phospho S251)抗体(ab56151)

概述

  • 产品名称
    Anti-REC8 (phospho S251)抗体
    参阅全部 REC8 一抗
  • 描述
    兔多克隆抗体to REC8 (phospho S251)
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Recombinant fragment
    预测可用于: Mouse, Rat
  • 免疫原

    A synthetic phospho-peptide (phospho-serine 251) corresponding to N-terminal residues of mouse REC8.

性能

应用

Our Abpromise guarantee covers the use of ab56151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 67 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.

靶标

  • 功能
    Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.
  • 组织特异性
    Expressed in testis and thymus.
  • 序列相似性
    Belongs to the rad21 family.
  • 翻译后修饰
    Phosphorylated.
  • 细胞定位
    Nucleus. Chromosome. In meiotic chromosomes, localized along axial elements in prophase from the leptotene to diplotene stages. At later prophase stages, diakinesis and metaphase I, localized along interstitial axes of chromosomes including both centromere and arm regions. No longer detected in arm regions in anaphase I but persists on centromere regions until metaphase II.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cohesin Rec8p antibody
    • Human homolog of rad21 S. pombe antibody
    • Meiotic recombination and sister chromatid cohesion phosphoprotein of the rad21p family antibody
    • Meiotic recombination protein REC8 homolog antibody
    • Meiotic recombination protein REC8-like 1 antibody
    • rec8 antibody
    • REC8 homolog (yeast) antibody
    • REC8 like 1 antibody
    • REC8_HUMAN antibody
    • Rec8p antibody
    • Recombination and sister chromatid cohesion protein homolog antibody
    see all

实验方案

Anti-REC8 (phospho S251) antibody (ab56151)参考文献

ab56151 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH 3T3)
Specification
NIH 3T3
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X100 in PBS
Username

Dr. Kirk Mcmanus

Verified customer

提交于 Jan 10 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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