概述

  • 产品名称Anti-REC8抗体
    参阅全部 REC8 一抗
  • 描述
    兔多克隆抗体to REC8
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse REC8.

    (Peptide available as ab38371.)

  • 阳性对照
    • IHC-P: FFPE mouse testis normal

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab38372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.
  • 组织特异性Expressed in testis and thymus.
  • 序列相似性Belongs to the rad21 family.
  • 翻译后修饰Phosphorylated.
  • 细胞定位Nucleus. Chromosome. In meiotic chromosomes, localized along axial elements in prophase from the leptotene to diplotene stages. At later prophase stages, diakinesis and metaphase I, localized along interstitial axes of chromosomes including both centromere and arm regions. No longer detected in arm regions in anaphase I but persists on centromere regions until metaphase II.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cohesin Rec8p antibody
    • Human homolog of rad21 S. pombe antibody
    • Meiotic recombination and sister chromatid cohesion phosphoprotein of the rad21p family antibody
    • Meiotic recombination protein REC8 homolog antibody
    • Meiotic recombination protein REC8-like 1 antibody
    • rec8 antibody
    • REC8 homolog (yeast) antibody
    • REC8 like 1 antibody
    • REC8_HUMAN antibody
    • Rec8p antibody
    • Recombination and sister chromatid cohesion protein homolog antibody
    see all

Anti-REC8 antibody 图像

  • IHC image of REC-8 staining in a section of formalin fixed, paraffin embedded normal mouse testis and normal mouse liver performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38372, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-REC8 antibody (ab38372)参考文献

This product has been referenced in:
  • Hu MW  et al. Scaffold subunit Aalpha of PP2A is essential for female meiosis and fertility in mice. Biol Reprod 91:19 (2014). Mouse . Read more (PubMed: 24899574) »
  • Labrador L  et al. Chromosome movements promoted by the mitochondrial protein SPD-3 are required for homology search during Caenorhabditis elegans meiosis. PLoS Genet 9:e1003497 (2013). IHC-Fr ; Caenorhabditis elegans . Read more (PubMed: 23671424) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (adult testes)
Specification adult testes
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
Blocking step PBS, 3% BSA, 5% serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Oct 04 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH-3T3 #Ms Testis #F9 #PC12)
Loading amount 30 µg
Specification NIH-3T3 #Ms Testis #F9 #PC12
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Oct 29 2008

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH 3T3)
Specification NIH 3T3
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100 in PBS
Username

Dr. Kirk McManus

Verified customer

提交于 Dec 05 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"