概述

  • 产品名称Anti-REA抗体
    参阅全部 REA 一抗
  • 描述
    兔多克隆抗体to REA
  • 经测试应用适用于: ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residues 225 and 275 of human REA (NP_009204.1)

  • 阳性对照
    • HeLa, 293T and NIH3T3 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab71970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
IP Use at 1-4 µg/mg of lysate.

靶标

  • 功能Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases (By similarity). Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging.
  • 序列相似性Belongs to the prohibitin family.
  • 发展阶段Levels of expression in fibroblasts decrease heterogeneously during cellular aging.
  • 细胞定位Mitochondrion inner membrane. Cytoplasm. Nucleus. Also cytoplasmic and nuclear.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B cell receptor associated protein BAP37 antibody
    • B-cell receptor-associated protein BAP37 antibody
    • BAP antibody
    • Bap37 antibody
    • BCAP 37 antibody
    • D prohibitin antibody
    • D-prohibitin antibody
    • p22 antibody
    • Phb2 antibody
    • PHB2_HUMAN antibody
    • PNAS 141 antibody
    • Prohibitin 2 antibody
    • Prohibitin-2 antibody
    • Repressor of estrogen receptor activity antibody
    see all

Anti-REA antibody 图像

  • All lanes : Anti-REA antibody (ab71970) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 37 kDa
    Observed band size : 37 kDa
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab71970 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71970, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-REA antibody (ab71970)参考文献

This product has been referenced in:

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab71970.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"