概述

  • 产品名称Anti-REA抗体
    参阅全部 REA 一抗
  • 描述
    兔多克隆抗体to REA
  • 经测试应用适用于: WB, IP, ICC, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant fusion protein, corresponding to amino acids 88-299 of Human REA fused to (His)6.

性能

应用

Our Abpromise guarantee covers the use of ab15019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 33 kDa.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P 1/300. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases (By similarity). Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging.
  • 序列相似性Belongs to the prohibitin family.
  • 发展阶段Levels of expression in fibroblasts decrease heterogeneously during cellular aging.
  • 细胞定位Mitochondrion inner membrane. Cytoplasm. Nucleus. Also cytoplasmic and nuclear.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B cell receptor associated protein BAP37 antibody
    • B-cell receptor-associated protein BAP37 antibody
    • BAP antibody
    • Bap37 antibody
    • BCAP 37 antibody
    • D prohibitin antibody
    • D-prohibitin antibody
    • p22 antibody
    • Phb2 antibody
    • PHB2_HUMAN antibody
    • PNAS 141 antibody
    • Prohibitin 2 antibody
    • Prohibitin-2 antibody
    • Repressor of estrogen receptor activity antibody
    see all

Anti-REA antibody 图像

  • Anti-REA antibody (ab15019) at 1 µg/ml + Human brain whole tissue lysate at 50 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG (H+L) polyclonal at 3000 mg/ml
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 33 kDa
    Observed band size : 33 kDa


    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview



  • Predicted band size : 33 kDa
    3T3 nuclear extract probed with ab15019 (1ug/ml). Proteins were visualized using an IgG (HRP) polyclonal goat anti-rabbit secondary antibody. Chemiluminescence detection system was used. Arrow indicates REA (37kDa).
  • ab15019 at a 1/300 dilution staining REA in primary human breast cancer tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Tissue blocked with a 5% blocking inhibitor. The secondary was biotinylated.

Anti-REA antibody (ab15019)参考文献

This product has been referenced in:
  • Yoshimaru T  et al. Xanthohumol suppresses oestrogen-signalling in breast cancer through the inhibition of BIG3-PHB2 interactions. Sci Rep 4:7355 (2014). WB ; Human . Read more (PubMed: 25483453) »
  • Cao G  et al. Regulation of the epithelial Mg2+ channel TRPM6 by estrogen and the associated repressor protein of estrogen receptor activity (REA). J Biol Chem 284:14788-95 (2009). WB ; Mouse . Read more (PubMed: 19329436) »

See all 2 Publications for this product

Product Wall

Abreviews
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing
Sample Human Tissue lysate - whole (Brain)
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Sep 30 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse ES Cells)
Loading amount 60 µg
Specification Mouse ES Cells
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Mr. Utkarsh Kapoor

Verified customer

提交于 Dec 17 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Transformed embryonic kidney)
Loading amount 20 µg
Specification Transformed embryonic kidney
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Feb 22 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (HEK 293 mitochondrial enriched fraction)
Loading amount 20 µg
Specification HEK 293 mitochondrial enriched fraction
Gel Running Conditions Reduced Denaturing (4-12% Bis Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. Sarah Ronnebaum

Verified customer

提交于 May 19 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (PRIMARY HUMAN BREAST INVASIVE CANCERS)
Specification PRIMARY HUMAN BREAST INVASIVE CANCERS
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: CC1 (VENTANA ANTIGEN RETRIEVAL BUFFER) pH 8.0
Permeabilization No
Blocking step VENTANA BLOCKING INHIBITOR as blocking agent for 12 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Dr. George Skliris

Verified customer

提交于 Jul 13 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"