概述

  • 产品名称Anti-RDM1抗体
    参阅全部 RDM1 一抗
  • 描述
    兔多克隆抗体to RDM1
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from an internal sequence of Human RDM1.

  • 阳性对照
    • Paraffin embedded Human colon carcinoma tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49.1% PBS, 0.88% Sodium chloride, 50% Glycerol
    Note: PBS is without Mg2+ and Ca2+.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab111139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能May confer resistance to the antitumor agent cisplatin. Binds to DNA and RNA.
  • 组织特异性Expressed in testis.
  • 序列相似性Contains 1 RRM (RNA recognition motif) domain.
  • 结构域C-terminal half (amino acids 134-284) contains cytoplasmic retention domains as well as determinants involved in its stress-induced nucleolar accumulation.
  • 细胞定位Cytoplasm. Nucleus > PML body. Nucleus > Cajal body. Isoform 1 is predominantly cytoplasmic. Isoform 1 colocalized with nuclear promyelocytic leukemia (PML) and Cajal bodies (CB); this association with nuclear bodies is enhanced in response to proteotoxic stress; Nucleus. Cytoplasm. Nucleus > nucleolus. Isoform 3 and isoform 10 are predominantly nuclear and nucleolar. After treatment with proteasomal inhibitors and mild heat-shock stress, isoform 1, isoform 3, isoform 5, isoform 7, isoform 8 and isoform 10 are relocalized to the nucleolus as dot-like or irregular subnuclear structures. Isoform 1 colocalized with nuclear promyelocytic leukemia (PML) and Cajal bodies (CB) and this association with nuclear bodies is enhanced in response to proteotoxic stress. Isoform 3, but not isoform 1 and isoform 5, is relocalized in nucleolar caps during transcriptional arrest.
  • Information by UniProt
  • 数据库链接
  • 形式There are 11 isoforms produced by alternative promoter usage and alternative splicing.Isoform 1 = RDM1alpha; Long N-terminal form. Isoform 2 = DeltaN-RDM1alpha; Short N-terminal form. Isoform 3 = RDM1beta. Isoform 4 = DeltaN-RDM1beta. Isoform 5 = RDM1gamma. Isoform 6 = DeltaN-RDM1gamma. Isoform 7 = RDM1delta. Isoform 8 = RDM1epsilon. Isoform 9 = DeltaN-RDM1epsilon. Isoform 10 = RDM1zeta. Isoform 11 = DeltaN-RDM1zeta.
  • 别名
    • MGC33977 antibody
    • RAD52 homolog B antibody
    • RAD52 motif 1 antibody
    • RAD52 motif containing 1 antibody
    • RAD52 motif-containing protein 1 antibody
    • RAD52B antibody
    • RDM1 antibody
    • RDM1_HUMAN antibody
    see all

Anti-RDM1 antibody 图像

  • ab111139, at a 1/50 dilution, staining RDM1 in paraffin embedded Human colon carcinoma tissue by Immunohistochemistry. Image on the right was from tissue treated with the synthetic peptide.

Anti-RDM1 antibody (ab111139)参考文献

ab111139 has not yet been referenced specifically in any publications.

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