概述

  • 产品名称
  • 描述
    兔多克隆抗体to RCCD1
  • 经测试应用
    适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: PFIAVQPFPA LLDLPMGSDA VKASCGSRHT AVVTRTGELY TWGWGKYGQL GHEDTTSLDR PRRVEYFVDK QLQVKAVTCG PWNTYVYAVE KG, corresponding to amino acids 283-374 of Human RCCD1 .

  • 阳性对照
    • Human liver tissue; A431 cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.
WB 1/100 - 1/250.

靶标

图片

  • Lane 1 :
    Lanes 2 - 3 : Anti-RCCD1 antibody (ab122667)

    Lane 1 : Marker
    Lane 2 : HEK293T cell lysate - vector only transfected
    Lane 3 : HEK293T cell lysate - overexpressing RCCD1 with a C-terminal myc-DDK tag (~3.1kDa)
  • ab122667, at 1/50 dilution, staining RCCD1 in paraffin-embedded Human liver tissue by Immunohistochemistry
  • ab122667, at 4 µg/ml staining RCCD1 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).

文献

ab122667 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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