概述

  • 产品名称Anti-RCC2抗体
    参阅全部 RCC2 一抗
  • 描述
    兔多克隆抗体to RCC2
  • 经测试应用适用于: IHC-P, WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan, Bat, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residue 475 and the C-terminus (residue 522) of human RCC2. (NP_061185.1).

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

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应用

Our Abpromise guarantee covers the use of ab70788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1.
    • 序列相似性Contains 7 RCC1 repeats.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus > nucleolus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex.
    • Information by UniProt
    • 数据库链接
    • 别名
      • KIAA1470 antibody
      • Protein RCC2 antibody
      • RCC 2 antibody
      • RCC1 like protein TD 60 antibody
      • RCC1-like protein TD-60 antibody
      • RCC2 antibody
      • RCC2_HUMAN antibody
      • Regulator of chromosome condensation 2 antibody
      • TD60 antibody
      • Telophase disk protein of 60 kDa antibody
      see all

    Anti-RCC2 antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin basal cell carcinoma (left) and mouse CT26 colon carcinoma (right) tissues labelling RCC2 with ab70788 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
    • All lanes : Anti-RCC2 antibody (ab70788) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg


      Predicted band size : 56 kDa
      Observed band size : 56 kDa

    Anti-RCC2 antibody (ab70788)参考文献

    ab70788 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa)
    Loading amount 5 µg
    Specification HeLa
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 30 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (gastric)
    Loading amount 15 µg
    Specification gastric
    Gel Running Conditions Non-reduced Denaturing (4-12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    提交于 May 19 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"