Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.
序列相似性Contains 7 RCC1 repeats.
翻译后修饰N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
细胞定位Nucleus. Cytoplasm. Becomes dispersed throughout the cytoplasm during mitosis.
All lanes : Anti-RCC1 antibody (ab93784) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg Lane 2 : Whole cell lysate from HeLa cells at 15 µg Lane 3 : Whole cell lysate from HeLa cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique
Predicted band size : 45 kDa
Exposure time : 30 seconds
Anti-RCC1 antibody (ab93784)参考文献
has not yet been referenced specifically in any publications.