概述

  • 产品名称Anti-RCC1抗体
    参阅全部 RCC1 一抗
  • 描述
    兔多克隆抗体to RCC1
  • 经测试应用适用于: WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Horse, Guinea pig, Hamster, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide: MGTNYQLGTG QDEDAWSPVE MMGKQLENRV VLSVSSGGQH TVLLVKDKEQ S, corresponding to amino acids 371-421 of Human RCC1 (NP_001260.1).

  • 阳性对照
    • Whole cell lysate from HeLa cells and 293T cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Antibody was affinity purified using an epitope specific to RCC1 immobilized on a solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab93784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/2000 - 1/10000. Predicted molecular weight: 45 kDa.

    Is unsuitable for IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.
    • 序列相似性Contains 7 RCC1 repeats.
    • 翻译后修饰N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
    • 细胞定位Nucleus. Cytoplasm. Becomes dispersed throughout the cytoplasm during mitosis.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cell cycle regulatory protein antibody
      • CHC 1 antibody
      • CHC1 antibody
      • Chromosome condensation 1 antibody
      • Chromosome condensation protein 1 antibody
      • Guanine nucleotide releasing protein antibody
      • HERC2 antibody
      • Ran GEF antibody
      • RanGEF antibody
      • RCC 1 antibody
      • RCC1 antibody
      • RCC1 I antibody
      • RCC1_HUMAN antibody
      • Regulator of chromosome condensation 1 antibody
      • Regulator of chromosome condensation antibody
      • SHEP1 antibody
      • SNHG3 RCC1 antibody
      • SNHG3 RCC1 readthrough transcript antibody
      see all

    Anti-RCC1 antibody 图像

    • All lanes : Anti-RCC1 antibody (ab93784) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg

      Developed using the ECL technique

      Predicted band size : 45 kDa


      Exposure time : 30 seconds

    Anti-RCC1 antibody (ab93784)参考文献

    ab93784 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"