Anti-RCC1抗体(ab70749)
Key features and details
- Goat polyclonal to RCC1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RCC1抗体
参阅全部 RCC1 一抗 -
描述
山羊多克隆抗体to RCC1 -
宿主
Goat -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla -
免疫原
Synthetic peptide corresponding to Human RCC1 (N terminal).
Database link: P18754 -
阳性对照
- WB: Whole cell lysate from HeLa, 293T cells; IP: HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70749于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at 1-2 µg/mg of lysate.
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WB |
1/2000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
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说明 |
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IP
Use at 1-2 µg/mg of lysate. |
WB
1/2000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
靶标
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功能
Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly. -
序列相似性
Contains 7 RCC1 repeats. -
翻译后修饰
N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran. -
细胞定位
Nucleus. Cytoplasm. Becomes dispersed throughout the cytoplasm during mitosis. - Information by UniProt
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数据库链接
- Entrez Gene: 1104 Human
- Omim: 179710 Human
- SwissProt: P18754 Human
- Unigene: 469723 Human
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别名
- Cell cycle regulatory protein antibody
- CHC 1 antibody
- CHC1 antibody
see all
图片
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All lanes : Anti-RCC1 antibody (ab70749) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK-293T whole ell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 45 kDaChemiluminescence with an exposure time of 30 seconds.
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Detection of human RCC1 by western blot of immunoprecipitates.
Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Lane 1: Anti-RCC1 antibody ab70749 used for IP at 6 µg per reaction
Lane 2: Anti-RCC1 antibody, ab245594
Lane 3: IgG Control
For blotting immunoprecipitated RCC1, ab70749 was used at 1 µg/mL.
Detection: Chemiluminescence with an exposure time of 30 seconds.
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All lanes : Anti-RCC1 antibody (ab70749) at 1/2000 dilution
Lane 1 :Recombinant Human RCC1 protein (ab90625) at 0.01 µg
Lane 2 :Recombinant Human RCC1 protein (ab90625) at 0.1 µg
Secondary
All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution (Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP))
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Exposure time: 8 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab70749 尚未被引用在任何文献中。