概述

  • 产品名称
  • 描述
    兔多克隆抗体to RCC1
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic Peptide corresponding to N-terminal of human RCC1.

  • 阳性对照
    • Jurkat and A431 cells. Colon carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab36760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明
    IHC-P: 1/100 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 47 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.
    • 序列相似性
      Contains 7 RCC1 repeats.
    • 翻译后修饰
      N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
    • 细胞定位
      Nucleus. Cytoplasm. Becomes dispersed throughout the cytoplasm during mitosis.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cell cycle regulatory protein antibody
      • CHC 1 antibody
      • CHC1 antibody
      • Chromosome condensation 1 antibody
      • Chromosome condensation protein 1 antibody
      • Guanine nucleotide releasing protein antibody
      • HERC2 antibody
      • Ran GEF antibody
      • RanGEF antibody
      • RCC 1 antibody
      • RCC1 antibody
      • RCC1 I antibody
      • RCC1_HUMAN antibody
      • Regulator of chromosome condensation 1 antibody
      • Regulator of chromosome condensation antibody
      • SHEP1 antibody
      • SNHG3 RCC1 antibody
      • SNHG3 RCC1 readthrough transcript antibody
      see all

    图片

    • Ab36760 staining human RCC1 in human colon carcinoma by immunohistochemistry using formalin fixed, paraffin embedded tissue.

    文献

    ab36760 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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