概述

  • 产品名称Anti-RC74抗体
    参阅全部 RC74 一抗
  • 描述
    兔多克隆抗体to RC74
  • 经测试应用适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rabbit, Horse, Guinea pig, Cow, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 150 and 200 of human RC74 (NP_060720.1)

  • 阳性对照
    • HeLa cell nuclear extract.

性能

应用

Our Abpromise guarantee covers the use of ab70586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/10000 - 1/25000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
IP Use at 1-4 µg/mg of lysate.

靶标

Anti-RC74 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling RC74 with ab70586 at 1/5000 (0.2µg/ml). Detection: DAB.
  • Anti-RC74 antibody (ab70586) at 0.05 µg/ml + HeLa cell nuclear extract at 25 µg

    Predicted band size : 74 kDa
    Observed band size : 74 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • Immunoprecipitation of RC74 from HeLa cell nuclear extract (5 mg for IP) with ab70586 at 4 µg/5 mg extract for IP.
    Detection: Coomassie stain.
    The identity of the bands that are RC74 was verified by mass spectrometry.

Anti-RC74 antibody (ab70586)参考文献

ab70586 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab70586.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"