概述

  • 产品名称Anti-RBPMS抗体
    参阅全部 RBPMS 一抗
  • 描述
    兔多克隆抗体to RBPMS
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 170-219 (PASLHAQCFS PEAKPNTPVF CPLLQQIRFV SGNVFVTYQP TADQQRELPC) of human RBPMS (NP_001008712).

  • 阳性对照
    • MCF7 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab83090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Acts as a coactivator of transcriptional activity. Required to increase TGFB1/Smad-mediated transactivation. Acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity. Increases phosphorylation of SMAD2 and SMAD3 on their C-terminal SSXS motif, possibly through recruitment of TGFBR1. Promotes the nuclear accumulation of SMAD2, SMAD3 and SMAD4 proteins. Binds to poly(A) RNA.
  • 组织特异性Ubiquitously expressed, at various levels depending on the isoform and the tissue.
  • 序列相似性Contains 1 RRM (RNA recognition motif) domain.
  • 结构域The RRM domain is necessary for interaction with SMAD4. Both the RRM domain and the C-terminus are required for TGFB1/Smad-mediated transactivation activity.
  • 细胞定位Nucleus. Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ32971 antibody
    • Heart and RRM expressed sequence antibody
    • Hermes antibody
    • RBP MS antibody
    • RBP-MS antibody
    • Rbpms antibody
    • RBPMS_HUMAN antibody
    • RNA binding protein gene with multiple splicing antibody
    • RNA-binding protein with multiple splicing antibody
    see all

Anti-RBPMS antibody 图像

  • Anti-RBPMS antibody (ab83090) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

Anti-RBPMS antibody (ab83090)参考文献

ab83090 has not yet been referenced specifically in any publications.

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