Anti-RBPJK抗体- ChIP Grade (ab25949)

概述

  • 产品名称Anti-RBPJK抗体- ChIP Grade
    参阅全部 RBPJK 一抗
  • 描述
    兔多克隆抗体to RBPJK - ChIP Grade
  • 经测试应用适用于: IP, ChIP, EMSA, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human RBPJK.

    (Peptides available as ab26893)

  • 阳性对照
    • This antibody gave a positive signal in Human Pancreas tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab25949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use a concentration of 5 µg/ml.
ChIP Use at an assay dependent concentration. PubMed: 20661424
EMSA Use at an assay dependent concentration. PubMed: 21991380
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence.
  • 序列相似性Belongs to the Su(H) family.
    Contains 1 IPT/TIG domain.
  • 细胞定位Nucleus. Cytoplasm. Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm, down-regulating the Notch signaling pathway.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI843960 antibody
    • CBF 1 antibody
    • CBF-1 antibody
    • CBF1 antibody
    • IGKJRB1 antibody
    • J kappa recombination signal binding protein antibody
    • J kappa-recombination signal-binding protein antibody
    • KBF2 antibody
    • NY REN 30 antigen antibody
    • RBP J kappa antibody
    • RBP-J antibody
    • RBP-J kappa antibody
    • RBP-JK antibody
    • Rbpj antibody
    • RBPJK antibody
    • RBPSUH antibody
    • Recombining binding protein suppressor of hairless antibody
    • Renal carcinoma antigen NY-REN-30 antibody
    • SUH_HUMAN antibody
    see all

Anti-RBPJK antibody - ChIP Grade 图像

  • Anti-RBPJK antibody - ChIP Grade (ab25949) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 20 µg

    Secondary
    Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866) at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Additional bands at : 98 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab25949 overnight at 4°C. Antibody binding was detected using an anti-rabbit IgG VHH single domain antibody conjugated to HRP (ab191866), and visualised using ECL development solution ab133406.

  • RBPJK was immunoprecipitated using 0.5mg PANC1 whole cell extract, 5µg of Rabbit polyclonal to RBPJK and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, PANC1 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab25949.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 56kDa, non specific bands -50kDa: This extra band is heavy chain contamination; RBPJK

  • All lanes : Anti-RBPJK antibody - ChIP Grade (ab25949) at 1/500 dilution

    Lane 1 : Mouse myoblast C2C12 whole cell lysate at 50 µg
    Lane 2 : Mouse myoblast C2C12 whole cell lysate at 75 µg

    Secondary
    HRP-conjugated Goat anti-rabbit at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 56 kDa


    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by JK Farmaha

    See Abreview

Anti-RBPJK antibody - ChIP Grade (ab25949)参考文献

This product has been referenced in:
  • Kumar V  et al. Notch and NF-kB signaling pathways regulate miR-223/FBXW7 axis in T-cell acute lymphoblastic leukemia. Leukemia N/A:N/A (2014). Read more (PubMed: 24727676) »
  • Zhang Y  et al. Mastermind-like transcriptional co-activator-mediated Notch signaling is indispensable for maintaining conjunctival epithelial identity. Development 140:594-605 (2013). ChIP . Read more (PubMed: 23293291) »

See all 5 Publications for this product

Product Wall

Thank you for taking the time to confirm this and sending us the qPCR results. The details you have kindly provided will provide us with vital information for our monitoring of product quality. It is regrettable the results have not been succes...

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Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (Mouse myoblast C2C12 cell line)
Specification Mouse myoblast C2C12 cell line
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

提交于 Oct 29 2013

I am very pleased to hear you would like to accept our offer and test ab25949 in mouse. Please order the antibody online if you prefer, and have the item deilvered to your Institute. This code will give you 1 free primary antibody before the expiration...

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Thank you very much for your interest in ab25949

To our knowledge, ab29549 has not been tested in mouse. Therefore, I can offer a discount off a future purchase if you buy ab25949 now, test it in mouse and submit feedback to us in the form o...

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Thank you for confirming this information and for your help and cooperation with this case. As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order. As usual ...

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Thank you for taking the time to contact us. I am sorry to hear the customer has had difficulty obtaining satisfactory results from this Anti-RBPJK antibody. I appreciate the time spent in the laboratory and understand the customer's concerns. It i...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Sample Human Cell (Human umbilical vein endothelial cells)
Specification Human umbilical vein endothelial cells
Fixative Paraformaldehyde
Permeabilization Yes - 1% Triton X-100
Username

Abcam user community

Verified customer

提交于 Apr 14 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"