概述

  • 产品名称Anti-RBPJK抗体
    参阅全部 RBPJK 一抗
  • 描述
    兔多克隆抗体to RBPJK
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Zebrafish
  • 免疫原

    Recombinant fragment, corresponding to a sequence within amino acids 258-498 of Human RBPJK (NP_005340)

  • 阳性对照
    • 293T, A431, HeLa, MOLT4 and Raji cells

性能

应用

Our Abpromise guarantee covers the use of ab97524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 56 kDa.
ICC/IF 1/100 - 1/200.

靶标

  • 功能Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence.
  • 序列相似性Belongs to the Su(H) family.
    Contains 1 IPT/TIG domain.
  • 细胞定位Nucleus. Cytoplasm. Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm, down-regulating the Notch signaling pathway.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI843960 antibody
    • CBF 1 antibody
    • CBF-1 antibody
    • CBF1 antibody
    • IGKJRB1 antibody
    • J kappa recombination signal binding protein antibody
    • J kappa-recombination signal-binding protein antibody
    • KBF2 antibody
    • NY REN 30 antigen antibody
    • RBP J kappa antibody
    • RBP-J antibody
    • RBP-J kappa antibody
    • RBP-JK antibody
    • Rbpj antibody
    • RBPJK antibody
    • RBPSUH antibody
    • Recombining binding protein suppressor of hairless antibody
    • Renal carcinoma antigen NY-REN-30 antibody
    • SUH_HUMAN antibody
    see all

Anti-RBPJK antibody 图像

  • Anti-RBPJK antibody (ab97524) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 56 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97524 at 1/100 dilution. Lower panel is merged with DNA probe.

Anti-RBPJK antibody (ab97524)参考文献

ab97524 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Cartilage)
Specification Cartilage
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 19°C
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提交于 Jan 26 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"