- 数据表
- 具体的参考文献 (2)
- 实验方案
概述
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产品名称Anti-RBPJK抗体
参阅全部 RBPJK 一抗 -
描述兔多克隆抗体to RBPJK
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宿主Rabbit
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特异性ab33065 appears to recognise a band at approximately 56 kDa in pancreatic tissue corresponding to RBPJK. However, ab33065 also recognises a band at 35 kDa, which may be due to non-specific binding.
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经测试应用适用于: IHC-P, EMSA, WBmore details
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种属反应性与反应: Human
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免疫原
Synthetic peptide derived from within residues 300 - 400 of Human RBPJK.
(Peptide available as ab26982.)
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阳性对照
- This antibody gave a positive signal in Human Pancreas tissue lysate in both Western blot and Immunohistochemistry.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Related Products
应用
Our Abpromise guarantee covers the use of ab33065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/250. | |
| EMSA | Use at an assay dependent concentration. PubMed: 21991380 | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
靶标
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功能Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence.
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序列相似性Belongs to the Su(H) family.
Contains 1 IPT/TIG domain. -
细胞定位Nucleus. Cytoplasm. Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm, down-regulating the Notch signaling pathway.
- Information by UniProt
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数据库链接
- Entrez Gene: 3516 Human
- Omim: 147183 Human
- SwissProt: Q06330 Human
- Unigene: 479396 Human
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别名
- AI843960 antibody
- AOS3 antibody
- CBF 1 antibody
see all
图片
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Western blot - Anti-RBPJK antibody (ab33065)Anti-RBPJK antibody (ab33065) at 1 µg/ml (Pancreas (Human) Tissue Lysate )
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RBPJK antibody (ab33065)This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.ab33065 staining RBPJK in human pancreatic tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/250 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.
文献
This product has been referenced in:
- Nagao H et al. RBPJ is a novel target for rhabdomyosarcoma therapy. PLoS One 7:e39268 (2012). WB ; Human . Read more (PubMed: 22792167) »
- Bartels SJ et al. A SILAC-Based Screen for Methyl-CpG Binding Proteins Identifies RBP-J as a DNA Methylation and Sequence-Specific Binding Protein. PLoS One 6:e25884 (2011). EMSA ; Human . Read more (PubMed: 21991380) »
客户评价及客户问答
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