概述

  • 产品名称
    Anti-RBPJK抗体
    参阅全部 RBPJK 一抗
  • 描述
    兔多克隆抗体to RBPJK
  • 宿主
    Rabbit
  • 特异性
    ab33065 appears to recognise a band at approximately 56 kDa in pancreatic tissue corresponding to RBPJK. However, ab33065 also recognises a band at 35 kDa, which may be due to non-specific binding.
  • 经测试应用
    适用于: IHC-P, EMSA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from within residues 300 - 400 of Human RBPJK.

    参阅Abcam的专有抗源政策

    (Peptide available as ab26982.)

  • 阳性对照
    • This antibody gave a positive signal in Human Pancreas tissue lysate in both Western blot and Immunohistochemistry.

性能

应用

Our Abpromise guarantee covers the use of ab33065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/250.
EMSA Use at an assay dependent concentration. PubMed: 21991380
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).

靶标

  • 功能
    Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence.
  • 序列相似性
    Belongs to the Su(H) family.
    Contains 1 IPT/TIG domain.
  • 细胞定位
    Nucleus. Cytoplasm. Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm, down-regulating the Notch signaling pathway.
  • Information by UniProt
  • 数据库链接
    • 别名
      • AI843960 antibody
      • AOS3 antibody
      • CBF 1 antibody
      • CBF-1 antibody
      • CBF1 antibody
      • csl antibody
      • IGKJRB antibody
      • IGKJRB1 antibody
      • J kappa recombination signal binding protein antibody
      • J kappa-recombination signal-binding protein antibody
      • KBF2 antibody
      • NY REN 30 antigen antibody
      • RBP J antibody
      • RBP J kappa antibody
      • RBP JK antibody
      • RBP-J antibody
      • RBP-J kappa antibody
      • RBP-JK antibody
      • Rbpj antibody
      • RBPJK antibody
      • RBPSUH antibody
      • recombination signal binding protein for immunoglobulin kappa J region antibody
      • Recombining binding protein suppressor of hairless antibody
      • Renal carcinoma antigen NY-REN-30 antibody
      • SUH antibody
      • SUH_HUMAN antibody
      see all

    图片

    • Western blot - Anti-RBPJK antibody (ab33065)
      Western blot - Anti-RBPJK antibody (ab33065)
      Anti-RBPJK antibody (ab33065) at 1 µg/ml (Pancreas (Human) Tissue Lysate )
      Secondary
      Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 56 kDa
      Observed band size: 56 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPJK antibody (ab33065)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPJK antibody (ab33065)This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.

      ab33065 staining RBPJK in human pancreatic tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/250 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.

      See Abreview

    文献

    This product has been referenced in:
    • Nagao H  et al. RBPJ is a novel target for rhabdomyosarcoma therapy. PLoS One 7:e39268 (2012). WB ; Human . Read more (PubMed: 22792167) »
    • Bartels SJ  et al. A SILAC-Based Screen for Methyl-CpG Binding Proteins Identifies RBP-J as a DNA Methylation and Sequence-Specific Binding Protein. PLoS One 6:e25884 (2011). EMSA ; Human . Read more (PubMed: 21991380) »

    See all 2 Publications for this product

    发表研究结果有使用 ab33065？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

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    1 Review or Q&A

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RBPJK antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (pancreas)
    Specification
    pancreas
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
    Permeabilization
    No
    Blocking step
    5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Read More
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    Username

    Mr. Antibody Solutions

    Verified customer

    提交于 Jan 22 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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