概述

  • 产品名称Anti-RBP4抗体[EP3657]
    参阅全部 RBP4 一抗
  • 描述
    兔单克隆抗体[EP3657] to RBP4
  • 经测试应用适用于: WB, ICC/IFmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human RBP4 aa 100-200.

  • 阳性对照
    • WB: Human plasma, HepG2 cell lysates, Human fetal kidney, Mouse serum and Rat serum. ICC/IF: HeLa cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

性能

应用

Our Abpromise guarantee covers the use of ab109193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 功能Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.
    • 疾病相关Defects in RBP4 are a cause of retinol-binding protein deficiency (RBP deficiency) [MIM:180250]. This condition causes night vision problems. It produces a typical 'fundus xerophthalmicus', featuring a progressed atrophy of the retinal pigment epithelium.
    • 序列相似性Belongs to the calycin superfamily. Lipocalin family.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • OTTHUMP00000020114 antibody
      • OTTHUMP00000020115 antibody
      • OTTHUMP00000020116 antibody
      • Plasma retinol binding protein 4 antibody
      • Plasma retinol-binding protein antibody
      • Plasma retinol-binding protein(1-176) antibody
      • PRBP antibody
      • PRO2222 antibody
      • RBP antibody
      • RBP4 antibody
      • RDCCAS antibody
      • RET4_HUMAN antibody
      • Retinol binding protein 4 antibody
      • Retinol binding protein 4 interstitial antibody
      • Retinol binding protein 4 plasma antibody
      see all

    Anti-RBP4 antibody [EP3657] 图像

    • All lanes : Anti-RBP4 antibody [EP3657] (ab109193) at 1/5000 dilution

      Lane 1 : HepG2 (human hepatocellular carcinoma) whole cell lysate
      Lane 2 : Human fetal kidney

      Lysates/proteins at 20 µg per lane.

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 23 kDa
      Observed band size : 23 kDa
      Blocking and diluting buffer 5% NFDM/TBST
    • Anti-RBP4 antibody [EP3657] (ab109193) at 1/5000 dilution + Mouse serum at 20 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 23 kDa
      Observed band size : 23 kDa
      Blocking and diluting buffer 5% NFDM/TBST
    • Anti-RBP4 antibody [EP3657] (ab109193) at 1/5000 dilution + Rat serum at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 23 kDa
      Observed band size : 23 kDa
      Blocking and diluting buffer 5% NFDM/TBST
    • All lanes : Anti-RBP4 antibody [EP3657] (ab109193) at 1/1000 dilution (un-purified)

      Lane 1 : Human plasma lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 23 kDa
      Observed band size : 23 kDa
    • Immunocytochemistry/immunofluorescence staining of 100% methanol fixed; 0.1% triton X 100 permeabilized HeLa (human cervix adenocarcinoma) cells with purified ab109193 at dilution of 1/100. The secondary antibody used was Alexa Fluor® 488; goat anti-rabbit IgG (ab150077) at a dilution of 1/1000. Nucleus was counter-stained with DAPI (blue). ab7291, a mouse anti-tubulin antibody (1/1000) was used to stain tubulin along with ab150120 (AlexaFluor®594 goat anti-mouse secondary, 1/1000) shown in the top right hand panel. The negative controls are shown in the bottom middle and right hand panels- for negative control 1 rabbit primary antibody and anti-mouse secondary antibody (ab150120) was used. For negative control 2 mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) was used.

    • Immunofluorescent analysis of HeLa cells labelling RBP4 with un-purified ab109193 at a dilution of 1/100.

    Anti-RBP4 antibody [EP3657] (ab109193)参考文献

    This product has been referenced in:
    • Kumar S  et al. Smoking accelerates pancreatic cancer progression by promoting differentiation of MDSCs and inducing HB-EGF expression in macrophages. Oncogene N/A:N/A (2014). WB ; Mouse . Read more (PubMed: 24909166) »

    See 1 Publication for this product

    Product Wall


    I am happy to hear you have already reduced, denatured, and protected your samples from proteases. The only other attempt that I think is worth your time is running a WB with mouse serum and kidney lysates, especially since the wildtype levels of ...

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    Thank you for contacting Abcam and providing your protocol information.

    I am sorry to hear that you have been experiencing issues when using this antibody. This product is covered by our Abpromise guarantee. According to Abpromise guarantee, i...

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    Thank you for contacting us.
    ab109193 has not been tested for neutralisation and we have no empirical data to support the application at the moment.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need a...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"