Anti-RBM5抗体(ab69770)
Key features and details
- Rabbit polyclonal to RBM5
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RBM5抗体
参阅全部 RBM5 一抗 -
描述
兔多克隆抗体to RBM5 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide derived from an internal region of Human RBM5
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阳性对照
- Brain tissue; extracts from HUVEC cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab69770于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
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IHC-P |
Use at an assay dependent concentration.
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa). |
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Component of the spliceosome A complex. Regulates alternative splicing of a number of mRNAs. May modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. May both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes exclusion of exon 6 thereby producing a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, promotes exclusion of exon 9 thereby producing a catalytically active form of CASP2/Caspase-2 that induces apoptosis. -
组织特异性
Isoform 5 is widely expressed in normal tissues and is expressed at increased levels in T-leukemic cell lines. -
序列相似性
Belongs to the RBM5/RBM10 family.
Contains 1 C2H2-type zinc finger.
Contains 1 G-patch domain.
Contains 1 RanBP2-type zinc finger.
Contains 2 RRM (RNA recognition motif) domains. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 10181 Human
- Entrez Gene: 83486 Mouse
- Omim: 606884 Human
- SwissProt: P52756 Human
- SwissProt: Q91YE7 Mouse
- Unigene: 439480 Human
- Unigene: 259197 Mouse
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别名
- FLJ39876 antibody
- G15 antibody
- H37 antibody
see all
图片
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Left: human brain tissue stained with ab69770 at 1/50 dilution; Right: the antibody was preincubated with the immunizing peptide.
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All lanes : Anti-RBM5 antibody (ab69770) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells
Lane 2 : Extracts from HUVEC cells with immunizing peptide at 10 µg
Lysates/proteins at 15 µg per lane.
Predicted band size: 92 kDa
Observed band size: 92 kDa
Additional bands at: 31 kDa. We are unsure as to the identity of these extra bands.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab69770 被引用在 1 文献中.
- Martínez FP & Tang Q Identification of cellular proteins that interact with human cytomegalovirus immediate-early protein 1 by protein array assay. Viruses 6:89-105 (2014). PubMed: 24385082