概述

  • 产品名称Anti-RBM4抗体
    参阅全部 RBM4 一抗
  • 描述
    兔多克隆抗体to RBM4
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 304-366 of Human RBM4 (AAC51293)

  • 阳性对照
    • HepG2 whole cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 1 x PBS, pH 7.0
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab96658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Predicted molecular weight: 40 kDa.

靶标

  • 功能RNA-binding factor involved in multiple aspects of cellular processes like alternative splicing of pre-mRNA and translation regulation. Modulates alternative 5'-splice site and exon selection. Acts as a muscle cell differentiation-promoting factor. Activates exon skipping of the PTB pre-mRNA during muscle cell differentiation. Antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin. Binds to intronic pyrimidine-rich sequence of the TPM1 and MAPT pre-mRNAs. Required for the translational activation of PER1 mRNA in response to circadian clock. Binds directly to the 3'-UTR of the PER1 mRNA. Exerts a suppressive activity on Cap-dependent translation via binding to CU-rich responsive elements within the 3'UTR of mRNAs, a process increased under stress conditions or during myocytes differentiation. Recruits EIF4A1 to stimulate IRES-dependent translation initiation in respons to cellular stress. Associates to internal ribosome entry segment (IRES) in target mRNA species under stress conditions. Plays a role for miRNA-guided RNA cleavage and translation suppression by promoting association of EIF2C2-containing miRNPs with their cognate target mRNAs. Associates with miRNAs during muscle cell differentiation. Binds preferentially to 5'-CGCGCG[GCA]-3' motif in vitro.
  • 组织特异性Expressed in the cerebellum. Expressed in neurons and glial cells, including layers II neurons in the frontal cortex and CA1 pyramidal neurons in the hippocampus. Expressed in heart, liver, pancreas, skeletal muscle, placenta, primary fibroblasts and peripheral blood monocytes (at protein level). Ubiquitously expressed. Highly expressed in heart, placenta and skeletal muscle. Weakly expressed in pancreas, kidney, liver, lung and brain.
  • 序列相似性Contains 1 CCHC-type zinc finger.
    Contains 2 RRM (RNA recognition motif) domains.
  • 发展阶段Found to be expressed in fetal brain. Down-regulated in fetal Down syndrome (DS) brain.
  • 翻译后修饰Phosphorylation on Ser-309 is induced upon cell muscle differentiation (By similarity). Phosphorylated. Phosphorylated in vitro on Ser-309 by SRPK1. Phosphorylation on Ser-309 is induced upon cell stress signaling, which alters its subcellular localization and may modulate its activity on IRES-mediated mRNA translation.
  • 细胞定位Nucleus. Nucleus > nucleolus. Nucleus speckle. Cytoplasm. Cytoplasmic granule. Undergoes continuous nucleocytoplasmic shuttling. Upon nuclear import colocalizes with SR proteins in nuclear speckles. Arsenite stress-induced phosphorylation increases its subcellular relocalization from the nucleus to the cytoplasm and to cytoplasmic stress granules (SG) via a p38 MAPK signaling pathway. Primarily localized in nucleus and nucleoli under cell growth conditions and accumulated in the cytoplasm and cytoplasm perinuclear granules upon muscle cell differentiation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • dkfzp547k0918 antibody
    • hLark antibody
    • lark antibody
    • Lark homolog antibody
    • lark homologue antibody
    • mgc75138 antibody
    • RBM4 antibody
    • rbm4 lark antibody
    • RBM4_HUMAN antibody
    • RNA binding motif 4 antibody
    • RNA binding motif 4a antibody
    • RNA-binding motif protein 4 antibody
    • RNA-binding motif protein 4a antibody
    • RNA-binding protein 4 antibody
    see all

Anti-RBM4 antibody 图像

  • Anti-RBM4 antibody (ab96658) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 40 kDa

Anti-RBM4 antibody (ab96658)参考文献

ab96658 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Hep G2)
Loading amount 100000 cells
Specification Hep G2
Treatment 15 nM siRNA transfection for 72 hours
Gel Running Conditions Reduced Denaturing (4-12% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Shijian Zhang

Verified customer

提交于 Jul 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"