概述

  • 产品名称Anti-RBM35A抗体[27H12]
    参阅全部 RBM35A 一抗
  • 描述
    小鼠单克隆抗体[27H12] to RBM35A
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Horse, Cow, Dog, Human, Pig, Chimpanzee, Opossum
  • 免疫原

    Full length recombinant Mouse RBM35A with a proprietary tag (NP_918944.2).

  • 阳性对照
    • RBM35A transfected 293T cell extract
  • 常规说明Store undiluted; dilute only prior to immediate use.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明ab106585 is an IgG fraction antibody purified from tissue culture supernatant by Protein A chromatography followed by extensive dialysis against the buffer stated. The antibody is sterile filtered.
  • 克隆单克隆
  • 克隆编号27H12
  • 同种型IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab106585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 76 kDa.

靶标

Anti-RBM35A antibody [27H12] 图像

  • All lanes : Anti-RBM35A antibody [27H12] (ab106585) at 1/1000 dilution

    Lane 1 : GFP transfected 293T cell extract
    Lane 2 : RBM35A transfected 293T cell extract
    Lane 3 : RBM35B transfected 293T cell extract

    Lysates/proteins at 5 µg per lane.

    Secondary
    anti-mouse ECL at 1/10000 dilution
    Developed using the ECL technique

    Predicted band size : 76 kDa

Anti-RBM35A antibody [27H12] (ab106585)参考文献

This product has been referenced in:
  • Warzecha CC  et al. An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition. EMBO J 29:3286-300 (2010). Read more (PubMed: 20711167) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HEK293, HCT116)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 20 µg
Specification HEK293, HCT116
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Simon Boudreault

Verified customer

提交于 Oct 26 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"