概述

  • 产品名称Anti-RBM34抗体
    参阅全部 RBM34 一抗
  • 描述
    兔多克隆抗体to RBM34
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide, corresponding to a region between residues 75 and 125 of human RBM34 (CAI21936.1).

  • 阳性对照
    • HeLa whole cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab86788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 10 µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 49 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 序列相似性Belongs to the RRM RBM34 family.
      Contains 2 RRM (RNA recognition motif) domains.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus > nucleolus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • KIAA0117 antibody
      • Rbm34 antibody
      • RBM34_HUMAN antibody
      • RNA binding motif protein 34 antibody
      • RNA-binding motif protein 34 antibody
      • RNA-binding protein 34 antibody
      see all

    Anti-RBM34 antibody 图像

    • All lanes : Anti-RBM34 antibody (ab86788) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 49 kDa
      Observed band size : 49 kDa


      Exposure time : 10 seconds
    • Detection of RBM34 by Western Blot of Immunprecipitate.
      ab86788, at 0.4 µg/ml, staining RBM34 in HeLa whole cell lysate immunoprecipitated using ab86788 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 30 seconds.

    Anti-RBM34 antibody (ab86788)参考文献

    ab86788 has not yet been referenced specifically in any publications.

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