Synthetic peptide corresponding to a region between residues 370 and 430 of human RBM34 (CAI21936.1).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate buffer, pH 7-8
Concentration information loading...
Immunogen affinity purified
ab86787 was affinity purified using an epitope specific to RBM34 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
Use at 10 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Belongs to the RRM RBM34 family.
Contains 2 RRM (RNA recognition motif) domains.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus > nucleolus.
Information by UniProt
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM34 antibody (ab86787)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling RBM34 with ab86787 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - Anti-RBM34 antibody (ab86787)
All lanes : Anti-RBM34 antibody (ab86787) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Developed using the ECL technique. Predicted band size: 49 kDa Observed band size: 49 kDa Exposure time: 10 seconds
Immunoprecipitation - Anti-RBM34 antibody (ab86787)
Detection of RBM34 by Western Blot of Immunprecipitate.
ab86787 at 0.4 µg/ml staining RBM34 in HeLa whole cell lysate immunoprecipitated using ab86787 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"