Synthetic peptide, corresponding to amino acids 344-394 of Human RBM34
Human breast carcinoma atissue; NIH3T3 cell exrracts.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium azide Constituents: PBS, 0.88% Sodium chloride, 50% Glycerol Note: PBS (without Mg2+ and Ca2+)
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Immunogen affinity purified
ab110859 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/500 - 1/1000. Predicted molecular weight: 49 kDa.
Belongs to the RRM RBM34 family.
Contains 2 RRM (RNA recognition motif) domains.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus > nucleolus.
Information by UniProt
Anti-RBM34 antibody 图像
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RBM34 antibody (ab110859)
ab110859 at 1/50 staining RBM34 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry; left panel shows staining without immunizing peptide right panel shows staining with immunizing peptide.
Western blot - RBM34 antibody (ab110859)
All lanes : Anti-RBM34 antibody (ab110859) at 1/500 dilution Lane 1 : NIH3T3 cell extract Lane 2 : NIH3T3 cell extract with immunizing peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 49 kDa
Anti-RBM34 antibody (ab110859)参考文献
has not yet been referenced specifically in any publications.
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