Anti-RBM3抗体(ab91291)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to RBM3
  • 经测试应用
    适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide selected from the Center region of Human RBM3 conjugated to KLH (NP_006734).

  • 阳性对照
    • NIH3T3 cell lysate IF/ICC: HeLa cell line.

性能

应用

Our Abpromise guarantee covers the use of ab91291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
WB
ELISA
  • 应用说明
    ELISA: 1/1000.
    WB: 1/50 - 1/100. Predicted molecular weight: 17 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphoryaltion of translation initiation factors and active polysome formation.
    • 序列相似性
      Contains 1 RRM (RNA recognition motif) domain.
    • 翻译后修饰
      Arg-105 is dimethylated, probably to asymmetric dimethylarginine.
      Phosphorylated.
    • 细胞定位
      Nucleus. Cytoplasm. Cell projection > dendrite. Localizes in mRNA granules in dentrites.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 2600016C11Rik antibody
      • IS1 RNPL antibody
      • MGC105811 antibody
      • MGC118410 antibody
      • OTTHUMP00000025800 antibody
      • OTTHUMP00000025802 antibody
      • OTTMUSP00000019634 antibody
      • OTTMUSP00000019635 antibody
      • OTTMUSP00000019636 antibody
      • Putative RNA binding protein 3 antibody
      • Putative RNA-binding protein 3 antibody
      • Rbm3 antibody
      • RBM3_HUMAN antibody
      • RNA binding motif (RNP1, RRM) protein 3 antibody
      • RNA binding motif protein 3 antibody
      • RNA-binding motif protein 3 antibody
      • RNA-binding protein 3 antibody
      • RNPL antibody
      • RP23-27I6.7 antibody
      see all

    图片

    • ICC/IF image of ab91291 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91291, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Anti-RBM3 antibody (ab91291) at 1/50 dilution + NIH3T3 cell lysate at 35 µg

      Predicted band size : 17 kDa

    文献

    ab91291 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (fibroblasts)
    Permeabilization
    Yes - 0.3% Triton X-100 in blocking buffer
    Specification
    fibroblasts
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Nov 20 2017

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    30 µg
    Specification
    HEK293
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 27 2017

    Thank you for contacting Abcam earlier today.

    I am sorry about the issues you have been having with ab118376 in western blotting. As we discussed I am sending you a vial of ab91291, as a free of charge replacement. Your new order number is *-*...

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    Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vi...

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    Thank you for contacting Abcam.

    I am sorry to hear of the difficulties that you have been experiencing using this product. Would you mind sending me the details of your protocol? Also extremely helpful are any images and steps that you may h...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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