Anti-RBM26抗体(ab74198)

概述

  • 产品名称Anti-RBM26抗体
    参阅全部 RBM26 一抗
  • 描述
    兔多克隆抗体to RBM26
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide corresponding to a region between residues 450 and 500 of human RBM26 (NP_071401.3)

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab74198 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 114 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 相关性RBM26 (RNA binding motif protein 26) bears two RNA recognition motif (RRM) domains and a C3H1-type zinc finger. The function remains unknown.
  • 数据库链接
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Anti-RBM26 antibody 图像

  • All lanes : Anti-RBM26 antibody (ab74198) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 114 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 seconds
  • Detection of RBM26 by Western Blot of Immunprecipitate.
    ab74198, at 1µg/ml, staining RBM26 in HeLa whole cell lysate immunoprecipitated using ab74198 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 1 second.

Anti-RBM26 antibody (ab74198)参考文献

ab74198 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (NSC34 cells)
Loading amount 10 µg
Specification NSC34 cells
Gel Running Conditions Reduced Denaturing (4-12% Nupage gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jul 08 2011

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