Mouse, Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide corresponding to a region between residues 200 and 250 of human RBM26 (NP_071401.3)
HeLa and 293T whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
ab74197 was affinity purified using an epitope specific to RBM26 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 114 kDa.
Use at 2-5 µg/mg of lysate.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
RBM26 (RNA binding motif protein 26) bears two RNA recognition motif (RRM) domains and a C3H1-type zinc finger. The function remains unknown.
acidic rich RS domain containing 2 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM26 antibody (ab74197)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue labelling RBM26 with ab74197 at 1/200 (1µg/ml). Detection: DAB.
Western blot - Anti-RBM26 antibody (ab74197)
All lanes :
Anti-RBM26 antibody (ab74197) at 0.04 µg/ml
Lane 1 :
HeLa whole cell lysate at 50 µg
Lane 2 :
HeLa whole cell lysate at 15 µg
Lane 3 :
HeLa whole cell lysate at 5 µg
Lane 4 :
293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size:
Observed band size:
125 kDa (
why is the actual band size different from the predicted?
Immunoprecipitation - Anti-RBM26 antibody (ab74197)
Detection of RBM26 by Western Blot of Immunprecipitate.
ab74197, at 1µg/ml, staining RBM26 in HeLa whole cell lysate immunoprecipitated using ab74197 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 1 second.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"