概述

  • 产品名称Anti-RBM19抗体
    参阅全部 RBM19 一抗
  • 描述
    兔多克隆抗体to RBM19
  • 经测试应用适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment corresponding to amino acids 377-486 of Human RBM19 (Q9Y4C8)

  • 阳性对照
    • Human stomach tissue, A431 cells; RT4 and U251 MG cell lysates; Human tonsil lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab122515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.
WB 1/100 - 1/500.

靶标

  • 功能Plays a role in embryo pre-implantation development.
  • 组织特异性Expressed in the crypts of Lieberkuhn of the intestine and in intestinal neoplasia (at protein level).
  • 序列相似性Belongs to the RRM MRD1 family.
    Contains 6 RRM (RNA recognition motif) domains.
  • 细胞定位Nucleus > nucleolus. Nucleus > nucleoplasm. Cytoplasm. Chromosome. In discrete foci distributed throughout the cytoplasm and nucleoplasm during the 4 to 8 cell stages and the morula stage, but not in the periphery of the nucleolar precursor body (NPB). During blastocyst development, becomes increasingly localized to the nucleolus and less to the cytoplasm. At the late blastocyst stage, localized predominantly in the nucleolus. Localized in the nucleolus during interphase and to the perichromosomal sheath during mitosis. Does not colocalize in the cytoplasm with GW182 in P-bodies. May translocate to the nucleolus upon early embryonic development (By similarity). Colocalizes with NPM1 during interphase. By late prophase, metaphase, anaphase and telophase, associates with the chromosome periphery. By telophase localizes to NPB.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1200009A02Rik antibody
    • AV302714 antibody
    • NPO antibody
    • Probable RNA-binding protein 19 antibody
    • RBM19 antibody
    • RBM19_HUMAN antibody
    • RNA-binding motif protein 19 antibody
    see all

Anti-RBM19 antibody 图像

  • All lanes : Anti-RBM19 antibody (ab122515) at 1/100 dilution

    Lane 1 : NIH-3T3 cell lysate (Mouse embryonic cells)
    Lane 2 : NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • ab122515 at 1/1000 dilution staining RBM19 in Paraffin-embedded Human Stomach tissue by Immunohistochemistry.
    Note: moderate cytoplasmic and nuclear positivity in glandular cells.
  • ab122515 at 4 µg/ml staining RBM19 in A431 cells by Immunofluorescence (green).
    Note: positivity in nucleus but not nucleoli & cytoplasm.
  • All lanes : Anti-RBM19 antibody (ab122515) at 1/250 dilution

    Lane 1 : RT4 lysate
    Lane 2 : U251 MG lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

Anti-RBM19 antibody (ab122515)参考文献

ab122515 has not yet been referenced specifically in any publications.

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