Recombinant fragment, corresponding to a region within amino acids 146-395 of Human Rbm15 (NP_073605).
WB: A431, H1299 and Hela cell lystes
IHC-P: HeLa cells
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Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 107 kDa.
1/100 - 1/200.
May be implicated in HOX gene regulation.
A chromosomal aberration involving RBM15 may be a cause of acute megakaryoblastic leukemia. Translocation t(1;22)(p13;q13) with MKL1. Although both reciprocal fusion transcripts are detected in acute megakaryoblastic leukemia (AMKL, FAB-M7), the RBM15-MKL1 chimeric protein has all the putative functional domains encoded by each gene and is the candidate oncogene.
Belongs to the RRM Spen family.
Contains 3 RRM (RNA recognition motif) domains. Contains 1 SPOC domain.
Information by UniProt
One twenty two protein antibody
One-twenty two protein 1 antibody
Western blot - Anti-Rbm15 antibody (ab96544)
Anti-Rbm15 antibody (ab96544) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 107 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Rbm15 antibody (ab96544)
ab96544, at a 1/200 dilution, staining Rbm15 in paraformaldehyde fixed HeLa cells by Immunofluorescence.
Lower image is merged with DNA probe.
has not yet been referenced specifically in any publications.
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