Anti-Rbm15/OTT抗体(ab70549)
Key features and details
- Rabbit polyclonal to Rbm15/OTT
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-Rbm15/OTT抗体
参阅全部 Rbm15/OTT 一抗 -
描述
兔多克隆抗体to Rbm15/OTT -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Orangutan, Elephant -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70549于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/2000 - 1/10000. Detects a band of approximately 107 kDa (predicted molecular weight: 107 kDa).
|
|
IP |
Use at 2-5 µg/mg of lysate.
|
说明 |
---|
WB
1/2000 - 1/10000. Detects a band of approximately 107 kDa (predicted molecular weight: 107 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
靶标
-
功能
May be implicated in HOX gene regulation. -
疾病相关
A chromosomal aberration involving RBM15 may be a cause of acute megakaryoblastic leukemia. Translocation t(1;22)(p13;q13) with MKL1. Although both reciprocal fusion transcripts are detected in acute megakaryoblastic leukemia (AMKL, FAB-M7), the RBM15-MKL1 chimeric protein has all the putative functional domains encoded by each gene and is the candidate oncogene. -
序列相似性
Belongs to the RRM Spen family.
Contains 3 RRM (RNA recognition motif) domains.
Contains 1 SPOC domain. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 64783 Human
- Omim: 606077 Human
- SwissProt: Q96T37 Human
- Unigene: 435947 Human
- Unigene: 708172 Human
-
别名
- One twenty two protein antibody
- One-twenty two protein 1 antibody
- OTT antibody
see all
图片
-
All lanes : Anti-Rbm15/OTT antibody (ab70549) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 107 kDa
Observed band size: 107 kDa
Exposure time: 3 minutes -
Detection of Human Rbm15/OTT by Western Blot of Immunprecipitate. ab70549 at 1µg/ml staining Rbm15/OTT in HeLa whole cell lysate immunoprecipitated by ab70549 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (5)
ab70549 被引用在 5 文献中.
- Horiuchi K et al. Wilms' tumor 1-associating protein complex regulates alternative splicing and polyadenylation at potential G-quadruplex-forming splice site sequences. J Biol Chem 297:101248 (2021). PubMed: 34582888
- Wang CY et al. PRC1 collaborates with SMCHD1 to fold the X-chromosome and spread Xist RNA between chromosome compartments. Nat Commun 10:2950 (2019). PubMed: 31270318
- Jin S et al. PRMT1-RBM15 axis regulates megakaryocytic differentiation of human umbilical cord blood CD34+cells. Exp Ther Med 15:2563-2568 (2018). PubMed: 29456659
- Knuckles P et al. Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor Rbm15/Spenito to the m6A machinery component Wtap/Fl(2)d. Genes Dev 32:415-429 (2018). PubMed: 29535189
- Horiuchi K et al. Identification of Wilms' tumor 1-associating protein complex and its role in alternative splicing and the cell cycle. J Biol Chem 288:33292-302 (2013). WB, ICC/IF ; Human . PubMed: 24100041