Anti-RBM14抗体(ab12325)
Key features and details
- Rabbit polyclonal to RBM14
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-RBM14抗体
参阅全部 RBM14 一抗 -
描述
兔多克隆抗体to RBM14 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide within Human RBM14 aa 150-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q96PK6 -
阳性对照
- Nuclear extract from HeLa cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
Purified using the peptide immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab12325于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/250 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 80 kDa. Prepare working dilutions immediately before use.
|
说明 |
---|
IHC-P
1/250 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Predicted molecular weight: 80 kDa. Prepare working dilutions immediately before use. |
靶标
-
功能
Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1 (PubMed:11443112). Regulates centriole biogenesis by suppressing the formation of aberrant centriolar protein complexes in the cytoplasm and thus preserving mitotic spindle integrity. Prevents the formation of the STIL-CENPJ complex (which can induce the formation of aberrant centriolar protein complexes) by interfering with the interaction of STIL with CENPJ (PubMed:25385835). -
组织特异性
Expressed in all tissues tested, including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes. -
序列相似性
Contains 2 RRM (RNA recognition motif) domains. -
细胞定位
Nucleus. Nucleus, nucleolus. Cytoplasm. In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles (PubMed:11790299). Cytoplasmic localization is crucial for its function in suppressing the formation of aberrant centriolar protein complexes (PubMed:25385835). - Information by UniProt
-
数据库链接
- Entrez Gene: 100526737 Human
- Entrez Gene: 10432 Human
- Omim: 612409 Human
- SwissProt: Q96PK6 Human
- Unigene: 523822 Human
- Unigene: 533712 Human
-
别名
- CoAA antibody
- Coactivator Activator antibody
- Paraspeckle protein 2 antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling RBM14 with ab12325 at 1/1000 (1µg/ml). Detection: DAB.
-
Detection of Human CoAA by Western Blot.
Samples: Nuclear extract (NE) from HeLa cells.
Antibody: ab12325 used at 0.3 mcg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Human CoAA by Western Blot. Samples: Nuclear extract (NE) from HeLa cells. Antibody: ab12325 used at 0.3 mcg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab12325 被引用在 2 文献中.
- Shiratsuchi G et al. RBM14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity. EMBO J 34:97-114 (2015). Human . PubMed: 25385835
- Li X et al. Co-activator activator (CoAA) prevents the transcriptional activity of Runt domain transcription factors. J Cell Biochem 108:378-87 (2009). PubMed: 19585539