概述

  • 产品名称Anti-RBM12抗体
    参阅全部 RBM12 一抗
  • 描述
    兔多克隆抗体to RBM12
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 882 and 932 of human RBM12 (NP_690051.1).

  • 阳性对照
    • Whole cell lysate from HeLa cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab72320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 97 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-RBM12 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling RBM12 with ab72320 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-RBM12 antibody (ab72320) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg


    Predicted band size : 97 kDa
    Observed band size : 117 kDa (why is the actual band size different from the predicted?)
  • Detection of RBM12 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab72320 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.

Anti-RBM12 antibody (ab72320)参考文献

ab72320 has not yet been referenced specifically in any publications.

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