Customer reviews and FAQs

概述

  • 产品名称Anti-RBM10抗体
    参阅全部 RBM10 一抗
  • 描述
    兔多克隆抗体to RBM10
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residue 880 and the C-terminus (residue 930) of human RBM10 (NP_005667.2)

  • 阳性对照
    • HeLa whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab72423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 104 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能May be involved in post-transcriptional processing, most probably in mRNA splicing. Binds to RNA homopolymers, with a preference for poly(G) and poly(U) and little for poly(A).
  • 疾病相关Defects in RBM10 are the cause of TARP syndrome (TARPS) [MIM:311900]. It is a disorder characterized by the Robin sequence (micrognathia, glossoptosis and cleft palate), talipes equinovarus and cardiac defects.
  • 序列相似性Contains 1 C2H2-type zinc finger.
    Contains 1 G-patch domain.
    Contains 1 RanBP2-type zinc finger.
    Contains 2 RRM (RNA recognition motif) domains.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus. In the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. Upon globally reducing RNA polymerase II transcription, the nuclear bodies enlarge and decrease in number. They occur closely adjacent to nuclear speckles or IGCs (interchromatin granule clusters) but coincide with TIDRs.
  • Information by UniProt
  • 数据库链接
    • 别名
      • DXS8237E antibody
      • G patch domain containing protein 9 antibody
      • G patch domain-containing protein 9 antibody
      • GPATC9 antibody
      • GPATCH9 antibody
      • HGNC9896 antibody
      • KIAA0122 antibody
      • MGC1132 antibody
      • MGC997 antibody
      • Rbm10 antibody
      • RBM10_HUMAN antibody
      • RNA binding motif protein 10 antibody
      • RNA binding protein 10 antibody
      • RNA binding protein S1-1 antibody
      • RNA-binding motif protein 10 antibody
      • RNA-binding protein 10 antibody
      • RNA-binding protein S1-1 antibody
      • S1-1 antibody
      • TARPS antibody
      • ZRANB5 antibody
      see all

    Anti-RBM10 antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM10 antibody (ab72423)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM10 antibody (ab72423)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling RBM10 with ab72423 at 1/200 (1µg/ml). Detection: DAB.
    • Western blot - RBM10 antibody (ab72423)
      Western blot - RBM10 antibody (ab72423)
      All lanes : Anti-RBM10 antibody (ab72423) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg


      Predicted band size : 104 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes
    • Immunoprecipitation - RBM10 antibody (ab72423)
      Immunoprecipitation - RBM10 antibody (ab72423)
      Detection of RBM10 by Western Blot of Immunprecipitate.
      ab72423 at 1µg/ml staining RBM10 in HeLa whole cell lysate immunoprecipitated using ab72423 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 10 seconds.

    Anti-RBM10 antibody (ab72423)参考文献

    This product has been referenced in:
    • Rodor J  et al. The RNA-binding landscape of RBM10 and its role in alternative splicing regulation in models of mouse early development. RNA Biol 14:45-57 (2017). WB, CLIP ; Mouse . Read more (PubMed: 27763814) »

    See 1 Publication for this product

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