- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-RBM10抗体
参阅全部 RBM10 一抗 -
描述兔多克隆抗体to RBM10
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宿主Rabbit
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经测试应用适用于: WB, IP, IHC-Pmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
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免疫原
Synthetic peptide corresponding to a region between residue 880 and the C-terminus (residue 930) of human RBM10 (NP_005667.2)
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阳性对照
- HeLa whole cell lysate.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明ab72423 was affinity purified using an epitope specific to RBM10 immobilized on solid support.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab72423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 104 kDa). | |
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能May be involved in post-transcriptional processing, most probably in mRNA splicing. Binds to RNA homopolymers, with a preference for poly(G) and poly(U) and little for poly(A).
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疾病相关Defects in RBM10 are the cause of TARP syndrome (TARPS) [MIM:311900]. It is a disorder characterized by the Robin sequence (micrognathia, glossoptosis and cleft palate), talipes equinovarus and cardiac defects.
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序列相似性Contains 1 C2H2-type zinc finger.
Contains 1 G-patch domain.
Contains 1 RanBP2-type zinc finger.
Contains 2 RRM (RNA recognition motif) domains. -
翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
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细胞定位Nucleus. In the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. Upon globally reducing RNA polymerase II transcription, the nuclear bodies enlarge and decrease in number. They occur closely adjacent to nuclear speckles or IGCs (interchromatin granule clusters) but coincide with TIDRs.
- Information by UniProt
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数据库链接
- Entrez Gene: 8241 Human
- Entrez Gene: 236732 Mouse
- Omim: 300080 Human
- SwissProt: P98175 Human
- SwissProt: Q99KG3 Mouse
- Unigene: 401509 Human
- Unigene: 279194 Mouse
- Unigene: 383632 Mouse
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别名
- DXS8237E antibody
- G patch domain containing protein 9 antibody
- G patch domain-containing protein 9 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM10 antibody (ab72423)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling RBM10 with ab72423 at 1/200 (1µg/ml). Detection: DAB. -
Western blot - RBM10 antibody (ab72423)All lanes : Anti-RBM10 antibody (ab72423) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 104 kDa
Observed band size: 120 kDa (why is the actual band size different from the predicted?)
Exposure time: 3 minutes -
Immunoprecipitation - RBM10 antibody (ab72423)Detection of RBM10 by Western Blot of Immunprecipitate.
ab72423 at 1µg/ml staining RBM10 in HeLa whole cell lysate immunoprecipitated using ab72423 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
文献
This product has been referenced in:
- Rodor J et al. The RNA-binding landscape of RBM10 and its role in alternative splicing regulation in models of mouse early development. RNA Biol 14:45-57 (2017). WB, CLIP ; Mouse . Read more (PubMed: 27763814) »
客户评价及客户问答
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