参阅全部 RbBP5 一抗
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human RbBP5.
(Peptide available as ab87828.)
- This antibody gave a positive signal in the following whole cell lysates: WERI; Y79; SW480; HEK293.
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- 存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
- 纯度Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).
- 功能As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation.
- 组织特异性Ubiquitously expressed.
- 序列相似性Contains 6 WD repeats.
- 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
- RBBP 5 antibody
- RBBP-5 antibody
- RBBP5 antibody
Anti-RbBP5 antibody 图像
Western blot - RbBP5 antibody (ab80074)
All lanes : Anti-RbBP5 antibody (ab80074) at 1 µg/ml
Lane 1 : WERI (Human Retinoblastoma) Whole Cell Lysate
Lane 2 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 59 kDa
Observed band size : 60,65 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
Anti-RbBP5 antibody (ab80074)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"