Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human RbBP5.
(Peptide available as
This antibody gave a positive signal in the following whole cell lysates: WERI; Y79; SW480; HEK293.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).
As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation.
Contains 6 WD repeats.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
RBBP 5 antibody
Western blot - RbBP5 antibody (ab80074)
All lanes :
Anti-RbBP5 antibody (ab80074) at 1 µg/ml
Lane 1 :
WERI (Human Retinoblastoma) Whole Cell Lysate
Lane 2 :
Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 3 :
SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 4 :
HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
60,65 kDa (
why is the actual band size different from the predicted?
Additional bands at:
100 kDa. We are unsure as to the identity of these extra bands.
RbBP5 contains a number of potential phosphorylation sites (SwissProt) which may explain the migration pattern observed. The antibody should cross-react with both isoform 1 (59-kDa) and isoform 2 (55-kDa) of RbBP5.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"