概述

  • 产品名称Anti-RbAp46抗体
    参阅全部 RbAp46 一抗
  • 描述
    兔多克隆抗体to RbAp46
  • 经测试应用适用于: ICC/IF, ICC, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human, Monkey
    预测可用于: Rat, Chicken, Xenopus laevis
  • 免疫原

    Synthetic peptide corresponding to Human RbAp46 aa 1-13.
    Sequence:

    MASKEMFEDTVEEC


    (Peptide available as ab5013)

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab3535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 22223663
ICC Use a concentration of 3.33 µg/ml.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 47.6 kDa).Can be blocked with RbAp46 peptide (ab5013).
IHC-P Use a concentration of 1 µg/ml.

靶标

  • 功能Core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the type B histone acetyltransferase (HAT) complex, which is required for chromatin assembly following DNA replication; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; and the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
  • 序列相似性Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
    Contains 7 WD repeats.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • G1/S transition control protein binding protein RbAp46 antibody
    • Histone acetyltransferase type B subunit 2 antibody
    • Histone binding protein RBBP7 antibody
    • Histone-binding protein RBBP7 antibody
    • MGC138867 antibody
    • MGC138868 antibody
    • Nucleosome remodeling factor subunit RBAP46 antibody
    • Nucleosome-remodeling factor subunit RBAP46 antibody
    • RBAP46 antibody
    • RBBP 7 antibody
    • RBBP-7 antibody
    • RBBP7 antibody
    • RBBP7_HUMAN antibody
    • Retinoblastoma binding protein 7 antibody
    • Retinoblastoma binding protein p46 antibody
    • Retinoblastoma-binding protein 7 antibody
    • Retinoblastoma-binding protein p46 antibody
    • Retinoblastoma-binding protein RbAp46 antibody
    see all

Anti-RbAp46 antibody 图像

  • ab3535 staining RbAp46 in Hela cells by Immunocytochemistry/ immunofluorescence. 

  • All lanes : Anti-RbAp46 antibody (ab3535) at 0.5 µg/ml

    Lane 1 : Cell extract prepared from DU-145 2 cells
    Lane 2 : Cell extract prepared from LNCaP cells
    Lane 3 : Cell extract prepared from HepG2 cells


    Predicted band size : 47.6 kDa
    Western blot of RbAp46 on 1) Du-148 2) LNCaP and 3) Hep-G2 cell extracts using ab3535.
  • Mouse embryonic fibroblasts (MEFs) were fixed in 4%PFA, permeabilised in PBS 1%BSA 0.1%TX-100 and incubated with 3.3 ug/ml ab3535 (anti-RbAp46 antibody) o/n. The antibody shows nuclear staining.

Anti-RbAp46 antibody (ab3535)参考文献

This product has been referenced in:
  • Teperek M  et al. Sperm and spermatids contain different proteins and bind distinct egg factors. Int J Mol Sci 15:16719-40 (2014). WB ; Frog . Read more (PubMed: 25244019) »
  • Ma P  et al. Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development. Proc Natl Acad Sci U S A 109:E481-9 (2012). WB, ICC/IF ; Mouse . Read more (PubMed: 22223663) »

See all 7 Publications for this product

Product Wall

Thank you very much for your interest in ab488. I am not sure whether you are interested in receiving more information about our testing program or if you are ready to receive the discount code, so I am sending all of the information about the program ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (SW480, colorectal cancer)
Loading amount 30 µg
Specification SW480, colorectal cancer
Gel Running Conditions Reduced Denaturing (12%/4%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 12 2008

Thank you for your patience. The originator of ab3535 has said that the antibody should not cross react with the RbAP48 protein. Please contact us again if you have any additional questions.

Thank you for your enquiry. For ab86 and ab488, the antibodies have not been tested against RbAp46 so we do not know whether or not the antibodies would react with it. I'm still waiting for information on ab3535 and will let you know when I receive.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"