概述

  • 产品名称Anti-Rb2 p130抗体
    参阅全部 Rb2 p130 一抗
  • 描述
    兔多克隆抗体to Rb2 p130
  • 特异性No reaction is observed against other related tumor supressor proteins.
  • 经测试应用适用于: ELISA, IHC-P, IP, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (Human) conjugated to KLH.

性能

应用

Our Abpromise guarantee covers the use of ab6545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/500 - 1/2000.
IHC-P 1/500 - 1/1000.
IP Use at an assay dependent concentration.
WB 1/200 - 1/1000. Predicted molecular weight: 128 kDa.

靶标

  • 相关性Two retinoblastoma related proteins, p107 and pRb2 / p130, which are structurally and functionally similar to the product of the retinoblastoma gene (pRb / p105), were cloned by taking advantage of their ability to bind transforming proteins of DNA tumor viruses through a particular region called the "pocket domain." Like pRb, both proteins play a fundamental role in growth control. These Rb family proteins were measured in a variety of lung neoplasms. The highest percentage of undetectable levels and the tightest inverse correlation with the histological grading and with PCNA expression in the most aggressive tumor types were found for pRb2 / p130, which may suggest an important role for this protein in the pathogenesis and progression of lung cancer.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • 130 kDa retinoblastoma associated protein antibody
    • FLJ26459 antibody
    • p130 antibody
    • PRB 2 antibody
    • PRB2 antibody
    • Rb 2 antibody
    • Rb2 antibody
    • RBL 2 antibody
    • RBL2 antibody
    • RBR 2 antibody
    • RBR2 antibody
    • Retinoblastoma like 2 antibody
    • Retinoblastoma like protein 2 antibody
    • Retinoblastoma related gene antibody
    see all

Anti-Rb2 p130 antibody 图像

  • ab6545 staining Rb2 p130 in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 4% paraformaldehyde in PBS at 4°C overnight, embedded in paraffin was and section a 7μm. Endogenous enzyme activity was quenched by 2% H2O2 in methanol (30 minutes). Sections were blocked wirh 2% BSA, 5% serum and 0.1% Trition X-100 in PBS. Samples were incubated with primary antibody (1/500 in blocking buffer). White arrow heads mark epithelial cells expressing high levels of protein in nuclear and perinuclear locations.

  • Immunohistochemical staining of mouse tissue using anti-pRb2 / p130 antiserum. 

    The staining shows the location of pRb2 / p130 in developing mouse tissue.

Anti-Rb2 p130 antibody (ab6545)参考文献

This product has been referenced in:
  • Herriges MJ  et al. Long noncoding RNAs are spatially correlated with transcription factors and regulate lung development. Genes Dev 28:1363-79 (2014). Read more (PubMed: 24939938) »
  • Cerezo A  et al. Absence of Caveolin-1 Increases Proliferation and Anchorage-Independent Growth by a Rac-Dependent, Erk-Independent Mechanism. Mol Cell Biol : (2009). WB ; Mouse . Read more (PubMed: 19620284) »

See all 3 Publications for this product

Product Wall

Thank you for contacting Abcam and for your help in finding the associated proteins on the phone.

I have had the chance to run the alignments of these proteins and have found that the homology between them is extremely poor. As such I do not...

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Thank you for your enquiry. I have enquired at the originator of the product but unfortunately, the immunogen sequence is not available, but this polyclonal antiserum recognizes epitopes in the C-terminus region of Rb2(p130). I hope this inform...

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Thank you for your enquriy. The sequence for ab2451 is as follows: DVLLKRLQDVVSERANH. I have contacted the source of ab6545 to find out the sequence. I will forward to you the information as soon as I receive it. Thank you for your patience. Have a gre...

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I was unfortunately unable to cannot find any supplier of this protein. The protein appears to be commonly expressed in most cell lines (including mouse 3T3 lines). You may wish to use cell lysates from these cells if you require a control tissue. ...

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We do not offer small samples for testing purposes, but rather our policy is that if a product does not work as specified on the datasheet we offer a replacement or refund.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"