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Our Abpromise guarantee covers the use of ab6545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/500 - 1/2000.|
|IHC-P||1/500 - 1/1000.|
|IP||Use at an assay dependent concentration.|
|WB||1/200 - 1/1000. Predicted molecular weight: 128 kDa.|
ab6545 staining Rb2 p130 in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 4% paraformaldehyde in PBS at 4°C overnight, embedded in paraffin was and section a 7μm. Endogenous enzyme activity was quenched by 2% H2O2 in methanol (30 minutes). Sections were blocked wirh 2% BSA, 5% serum and 0.1% Trition X-100 in PBS. Samples were incubated with primary antibody (1/500 in blocking buffer). White arrow heads mark epithelial cells expressing high levels of protein in nuclear and perinuclear locations.
Immunohistochemical staining of mouse tissue using anti-pRb2 / p130 antiserum.
The staining shows the location of pRb2 / p130 in developing mouse tissue.
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