Anti-Rb (phospho T821)抗体[24A7] (ab122893)

概述

  • 产品名称Anti-Rb (phospho T821)抗体[24A7]
    参阅全部 Rb 一抗
  • 描述
    小鼠单克隆抗体[24A7] to Rb (phospho T821)
  • 特异性ab122893 reacts with Human Rb phosphorylated at Thr821. Can detect endogenous levels of Rb phosphorylated at Thr821 in most cell lines.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide from a region containing phospho-Thr821 of Human Rb

  • 阳性对照
    • Crude extracts of Human lung carcinoma cell line H1299 transfected with plasmid expressing Myc-tagged wild-type Rb

性能

应用

Our Abpromise guarantee covers the use of ab122893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 106 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
  • 组织特异性Expressed in the retina.
  • 疾病相关Childhood cancer retinoblastoma
    Bladder cancer
    Osteogenic sarcoma
  • 序列相似性Belongs to the retinoblastoma protein (RB) family.
  • 结构域The Pocket domain binds to the threonine-phosphorylated domain C, thereby preventing interaction with heterodimeric E2F/DP transcription factor complexes.
  • 翻译后修饰Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis.
    N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1.
    Acetylation at Lys-873 and Lys-874 regulates subcellular localization, at least during keratinocytes differentiation.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • OSRC antibody
    • Osteosarcoma antibody
    • p105-Rb antibody
    • P105RB antibody
    • PP105 antibody
    • pp110 antibody
    • PPP1R130 antibody
    • pRb antibody
    • Prepro retinoblastoma associated protein antibody
    • Protein phosphatase 1 regulatory subunit 130 antibody
    • Rb antibody
    • RB_HUMAN antibody
    • RB1 antibody
    • RB1 gene antibody
    • Retinoblastoma 1 antibody
    • Retinoblastoma suspectibility protein antibody
    • Retinoblastoma-associated protein antibody
    see all

Anti-Rb (phospho T821) antibody [24A7] 图像

  • All lanes : Anti-Rb (phospho T821) antibody [24A7] (ab122893) at 1 µg/ml

    Lane 1 : Crude extracts of Human lung carcinoma cell line H1299 transfected with plasmid expressing Myc-tagged wild-type Rb and immuno-precipitated with anti-Myc antibody
    Lane 2 : Crude extracts of Human lung carcinoma cell line H1299 transfected with plasmid expressing Myc-tagged Rb (S795A) and immuno-precipitated with anti-Myc antibody
    Lane 3 : Crude extracts of Human lung carcinoma cell line H1299 transfected with plasmid expressing Myc-tagged Rb (T821A) and immuno-precipitated with anti-Myc antibody


    Predicted band size : 106 kDa

Anti-Rb (phospho T821) antibody [24A7] (ab122893)参考文献

ab122893 has not yet been referenced specifically in any publications.

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