RabMAb

Anti-Rb (phospho T356)抗体[EPR2153AY] (ab76298)

概述

  • 产品名称Anti-Rb (phospho T356)抗体[EPR2153AY]
    参阅全部 Rb 一抗
  • 描述
    兔单克隆抗体[EPR2153AY] to Rb (phospho T356)
  • 特异性The antibody only detects Rb phosphorylated on Threonine 356.
  • 经测试应用适用于: WB, IP, IHC-Pmore details
    不适用于: Flow Cyt or ICC/IF
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Rb.

  • 阳性对照
    • Jurkat cell lysate treated with FBS Human breast carcinoma tissue HeLa cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab76298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Detects a band of approximately 110 kDa (predicted molecular weight: 106 kDa).
IP 1/30.
IHC-P 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or ICC/IF.
  • 靶标

    • 功能Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
    • 组织特异性Expressed in the retina.
    • 疾病相关Childhood cancer retinoblastoma
      Bladder cancer
      Osteogenic sarcoma
    • 序列相似性Belongs to the retinoblastoma protein (RB) family.
    • 结构域The Pocket domain binds to the threonine-phosphorylated domain C, thereby preventing interaction with heterodimeric E2F/DP transcription factor complexes.
    • 翻译后修饰Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis.
      N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1.
      Acetylation at Lys-873 and Lys-874 regulates subcellular localization, at least during keratinocytes differentiation.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • OSRC antibody
      • Osteosarcoma antibody
      • p105-Rb antibody
      • P105RB antibody
      • PP105 antibody
      • pp110 antibody
      • PPP1R130 antibody
      • pRb antibody
      • Prepro retinoblastoma associated protein antibody
      • Protein phosphatase 1 regulatory subunit 130 antibody
      • Rb antibody
      • RB_HUMAN antibody
      • RB1 antibody
      • RB1 gene antibody
      • Retinoblastoma 1 antibody
      • Retinoblastoma suspectibility protein antibody
      • Retinoblastoma-associated protein antibody
      see all

    Anti-Rb (phospho T356) antibody [EPR2153AY] 图像

    • All lanes : Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298) at 1/4000 dilution

      Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) 2 day serum starved whole cell lysate.
      Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate.
      Lane 3 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate. Membrane incubated with phosphatase.

      Lysates/proteins at 15 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 106 kDa
      Observed band size : 106 kDa


      Exposure time : 1 minute
    • All lanes : Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298) at 1/2000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Jurkat cell lysate treated with FBS

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 106 kDa
      Observed band size : 106 kDa
    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab76298 at 1/100-1/250 dilution.
    • ab76298 showing positive staining in Lung adenocarcinoma tissue.

    • ab76298 showing positive staining in Ovarian carcinoma tissue.

    • ab76298 showing positive staining in Endometrial carcinoma tissue.

    • ab76298 showing positive staining in Normal tonsil tissue.

    • Dot blot analysis of Rb (phospho T356) phospho peptide (Lane 1), Rb Non-phospho peptide (Lane 2), labelling Rb (phospho T356) with ab76298 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    Anti-Rb (phospho T356) antibody [EPR2153AY] (ab76298)参考文献

    This product has been referenced in:
    • Li S  et al. MicroRNA-490-5p inhibits proliferation of bladder cancer by targeting c-Fos. Biochem Biophys Res Commun 441:976-81 (2013). WB ; Human . Read more (PubMed: 24220339) »

    See 1 Publication for this product

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