概述

  • 产品名称Anti-Rb (phospho S795)抗体
    参阅全部 Rb 一抗
  • 描述
    兔多克隆抗体to Rb (phospho S795)
  • 特异性This antibody detects endogenous levels of Rb only when phosphorylated at serine 795
  • 经测试应用适用于: ELISA, IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthesized phosphopeptide derived from human Rb around the phosphorylation site of serine 795 (P-S-SP-P-L)

  • 阳性对照
    • WB: Extracts from K562 cells. IHC-P: Human breast carcinoma tissue. ICC/IF: Formaldehyde fixed WERI cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab47474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 125 kDa (predicted molecular weight: 106 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
  • 组织特异性Expressed in the retina.
  • 疾病相关Childhood cancer retinoblastoma
    Bladder cancer
    Osteogenic sarcoma
  • 序列相似性Belongs to the retinoblastoma protein (RB) family.
  • 结构域The Pocket domain binds to the threonine-phosphorylated domain C, thereby preventing interaction with heterodimeric E2F/DP transcription factor complexes.
  • 翻译后修饰Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis.
    N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1.
    Acetylation at Lys-873 and Lys-874 regulates subcellular localization, at least during keratinocytes differentiation.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • OSRC antibody
    • Osteosarcoma antibody
    • p105-Rb antibody
    • P105RB antibody
    • PP105 antibody
    • pp110 antibody
    • PPP1R130 antibody
    • pRb antibody
    • Prepro retinoblastoma associated protein antibody
    • Protein phosphatase 1 regulatory subunit 130 antibody
    • Rb antibody
    • RB_HUMAN antibody
    • RB1 antibody
    • RB1 gene antibody
    • Retinoblastoma 1 antibody
    • Retinoblastoma suspectibility protein antibody
    • Retinoblastoma-associated protein antibody
    see all

Anti-Rb (phospho S795) antibody 图像

  • All lanes : Anti-Rb (phospho S795) antibody (ab47474) at 1/500 dilution

    Lane 1 : Extracts from K562 cells treated with 10% serum after 48 hours of starvation
    Lane 2 : Extracts from K562 cells

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 106 kDa
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue in the presence (right) and absence (left) of synthetic peptide, using ab47474 at a 1/50 dilution.
  • ICC/IF image of ab47474 stained WERI cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab47474 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Rb (phospho S795) antibody (ab47474)参考文献

This product has been referenced in:
  • Håland TW  et al. Simultaneous measurement of passage through the restriction point and MCM loading in single cells. Nucleic Acids Res 43:e150 (2015). WB ; Human . Read more (PubMed: 26250117) »
  • Zhu P  et al. GPER mediates the inhibitory actions of estrogen on adipogenesis in 3T3-L1 cells through perturbation of mitotic clonal expansion. Gen Comp Endocrinol 193C:19-26 (2013). Read more (PubMed: 23871778) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Cow Cell lysate - whole cell (aortic endothelial cells)
Loading amount 20 µg
Specification aortic endothelial cells
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Feb 02 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MCF-7)
Loading amount 5 µg
Specification MCF-7
Treatment CDK7 Inhibitors
Gel Running Conditions Reduced Denaturing (10)
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Dean Heathcote

Verified customer

提交于 Oct 28 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"