兔多克隆抗体to RB associated KRAB repressor
This antibody detects endogenous levels of total RB associated KRAB repressor protein.
A synthesized peptide derived from N-terminal of human RB associated KRAB repressor.
- Extracts from HepG2 cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 83 kDa).
May repress E2F-dependent transcription. May promote AR-dependent transcription.
Expressed in bone, brain, heart, kidney, liver, lung, pancreas and placenta.
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 16 C2H2-type zinc fingers.
Contains 1 KRAB domain.
Information by UniProt
- hRBaK antibody
- Hypothetical protein RBAK antibody
- RB associated KRAB zinc finger antibody
Western blot - Anti-RB associated KRAB repressor antibody (ab72609)
All lanes :
Anti-RB associated KRAB repressor antibody (ab72609) at 1/500 dilutionLane 1 :
Extracts from HepG2 cellsLane 2 :
Extracts from HepG2 cells plus 5µg immunizing peptide
Lysates/proteins at 5 µg per lane.Predicted band size:
83 kDaObserved band size:
85 kDa (why is the actual band size different from the predicted?
has not yet been referenced specifically in any publications.
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