Anti-RB associated KRAB repressor抗体(ab28487)

概述

  • 产品名称Anti-RB associated KRAB repressor抗体
    参阅全部 RB associated KRAB repressor 一抗
  • 描述
    兔多克隆抗体to RB associated KRAB repressor
  • 特异性ab28487 recognises RB associated KRAB repressor ((RBAK))
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from residues 447-496 of human RB associated KRAB repressor.

    .

  • 阳性对照
    • Raji cell lysate, human kidney.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab28487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/2500.
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 83 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能May repress E2F-dependent transcription. May promote AR-dependent transcription.
  • 组织特异性Expressed in bone, brain, heart, kidney, liver, lung, pancreas and placenta.
  • 序列相似性Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 16 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hRBaK antibody
    • Hypothetical protein RBAK antibody
    • RB associated KRAB zinc finger antibody
    • RB-associated KRAB repressor antibody
    • RB-associated KRAB zinc finger protein antibody
    • RBAK antibody
    • RBAK_HUMAN antibody
    • Zinc finger protein 769 antibody
    • ZNF 769 antibody
    • ZNF769 antibody
    see all

Anti-RB associated KRAB repressor antibody 图像

  • Anti-RB associated KRAB repressor antibody (ab28487) at 2 µg/ml (in 5% skim milk / PBS buffer) + Raji cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 83 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of RB associated KRAB repressor in paraffin embedded human kidney tissue slide using 4 µg/ml of ab28487. Arrows indicate positively labelled epithelial cells of the renal tubule.

Anti-RB associated KRAB repressor antibody (ab28487)参考文献

ab28487 has not yet been referenced specifically in any publications.

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