概述

  • 产品名称大鼠VEGF ELISA试剂盒
    参阅全部 VEGFA 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 2 pg/ml
  • 范围
    0.82 pg/ml - 200 pg/ml
  • 复检

    97 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 98.26 84% - 103%
    Serum 97.43 83% - 103%
    Plasma 96.42 85% - 104%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s VEGF Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat VEGF in serum, plasma, cellculture supernatants.

    This assay employs an antibody specific for rat VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • 组织特异性Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • 疾病相关Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • 序列相似性Belongs to the PDGF/VEGF growth factor family.
  • 细胞定位Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt
  • 别名
    • MGC70609
    • MVCD1
    • vascular endothelial growth factor
    • Vascular endothelial growth factor A
    • Vascular permeability factor
    • Vegf
    • VEGF A
    • VEGF-A
    • VEGF120
    • Vegfa
    • VEGFA_HUMAN
    • VPF
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Rat VEGF ELISA Kit 图像

  • VEGF measured in rat spleen and rat bone marrow showing quantity (pg) per mL of tested sample. Samples were diluted 9-27 fold.

  • VEGF measured in A10 cell culture supernatant and biological fluids showing quantity (pg) per mL of tested sample. RBL-1, PC12 and SV40LT-SIV cell culture supernatants were also assayed for rat VEGF and measured > 200 pg/mL.

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

实验方案

Rat VEGF ELISA Kit (ab100786)参考文献

This product has been referenced in:
  • Tzeng TF  et al. Consumption of Polyphenol-Rich Zingiber Zerumbet Rhizome Extracts Protects against the Breakdown of the Blood-Retinal Barrier and Retinal Inflammation Induced by Diabetes. Nutrients 7:7821-41 (2015). Read more (PubMed: 26389948) »
  • Zhao SL  et al. Mesenchymal stem cells with overexpression of midkine enhance cell survival and attenuate cardiac dysfunction in a rat model of myocardial infarction. Stem Cell Res Ther 5:37 (2014). Read more (PubMed: 24635859) »

See all 3 Publications for this product

Product Wall

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I look forward to receiving the resuls and order number for the ab100786 VEGF rat ELISA kit.

For the TNF kit ab46070, I think it is likely to be the sample that is the problem.However, I would be happy to...

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Thank you for your message. I look forward to receiving the further information regarding ab100786 VEGF Rat ELISA Kitso we can investigate this further for you.

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Thank you for your reply and for kindly providing the information.
I am sorry to confirm that the data in the attachments you have sent are not readable. The resolution of the image is not high enough to read the numbers properly, and it is faint....

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Thank you for taking the time to contact us. I am sorry to hear you have some concerns regarding the results from ab100786 VEGF Rat ELISA Kit.

I would like to reassure you that thiskit is covered by our 6 month guarantee. In the event that a...

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I am sorry to hear that you are experiencing difficulties with this kit. Can I confirm that you are using the protocol booklet information that comes with each new kit?

This is because there have been s...

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Thank you for contacting us.

First, I want to apologize to you for the delay in gathering this information. I hope this did not cause you any inconveniences.

In regards to using rat tissues in our SCF Mouse ELISA kit, there is no ...

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