大鼠TNF alpha酶联免疫吸附(ELISA)套装(without plates) (ab48419)

概述

  • 产品名称大鼠TNF alpha酶联免疫吸附(ELISA)套装(without plates)
    参阅全部 TNF alpha 试剂盒
  • 测试
    5 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 15 pg/ml
  • 范围
    31.25 pg/ml - 1000 pg/ml
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s TNFα Rat in vitro ELISA Set is designed for the quantitative measurement of Rat TNFα in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    The rat TNF alpha ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    This ELISA Set is intended to measure rat TNF alpha in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

     

    Recognizes both natural and recombinant Rat TNF alpha Range: 31.25 pg/ml - 1000 pg/ml Sensitivity < 15 pg/ml

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Reagents

性能

  • 相关性TNF-alpha is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF-alpha is a potent pyrogen causing fever by direct action or by stimulation of interleukin 1 secretion and is implicated in the induction of cachexia, under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • 细胞定位Cell membrane; single-pass type II membrane protein. Processed form: Secreted protein.
  • 别名
    • Cachectin
    • TNF
    • TNF alpha
    • TNFA
    • TNFSF2
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab48419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA
  • 应用说明ELISA: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Rat TNF alpha ELISA Set (without plates) 图像

    Rat TNF alpha ELISA Set (without plates) (ab48419)参考文献

    ab48419 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"