概述

  • 产品名称大鼠TNF alpha ELISA试剂盒
    参阅全部 TNF alpha 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 5.1%
    批次间
    样品 n Mean SD CV%
    Overall 7.1%
  • 样品类型
    Cell culture supernatant
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 0.015 ng/ml
  • 范围
    0.016 ng/ml - 1 ng/ml
  • 检测时间
    4h 30m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s TNF alpha Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TNF alpha in rat cell culture supernatants.

    A TNF alpha specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TNF alpha X specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TNF alpha captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • 疾病相关Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • 序列相似性Belongs to the tumor necrosis factor family.
  • 翻译后修饰The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 别名
    • APC1 protein
    • Cachectin
    • DIF
    • soluble form
    • Tnf
    • TNF a
    • TNF macrophage derived
    • TNF monocyte derived
    • TNF superfamily member 2
    • TNF-a
    • TNF-alpha
    • TNFA_HUMAN
    • TNFSF2
    • Tumor necrosis factor
    • Tumor necrosis factor alpha
    • Tumor necrosis factor ligand superfamily member 2
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

Rat TNF alpha ELISA Kit 图像

  • Representative Standard Curve using ab108913.

实验方案

Rat TNF alpha ELISA Kit (ab108913)参考文献

ab108913 has not yet been referenced specifically in any publications.

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