概述

  • 产品名称
    大鼠TIMP1 ELISA试剂盒
    参阅全部 TIMP1 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture extracts, Tissue Extracts
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 4 pg/ml
  • 范围
    4.1 pg/ml - 3000 pg/ml
  • 复检

    93 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture extracts 92.81 82% - 102%
    Tissue Extracts 93.42 84% - 103%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s TIMP1 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat TIMP1 in cell lysates and tissue lysates.

    This assay employs an antibody specific for Rat TIMP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat TIMP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab100783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative standard curve using ab100763

实验方案

文献

ab100783 has not yet been referenced specifically in any publications.

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